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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2013, Vol. 39 Issue (2): 167-172    DOI: 10.3785/j.issn.1008-9209.2012.11.068
论文     
菊米超临界CO2萃取物的气相色谱质谱分析及其抑菌活性
王春荣， 方程吉， 余庆青， 蒋鹏， 田薇*
浙江农林大学亚热带森林培育国家重点实验室培育基地，浙江 临安 311300
Gas chromatography and mass spectrometry (GCMS) analysis of supercritical CO2 extract of flower bud of Chrysanthemum indicum and its antibacterial activity. Journal of Zhejiang University
WANG Chunrong, FANG Chengji, YU Qingqing, JIANG Peng, TIAN Wei*
Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin’an, Zhejiang 311300, China
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摘要： 用气相色谱质谱(gas chromatographymass spectrometry, GCMS) 对通过超临界CO2萃取的菊米精油成分进行分析，并通过测定其抑菌圈直径判断其抑菌活性. 结果表明：经GCMS分析在菊米精油中共鉴定出28个相似度在70%以上的成分，以萜类及其衍生物为主，其中萜类主要为单萜和倍半萜，其衍生物主要为醇；菊米精油对金黄色葡萄球菌和大肠杆菌具有一定的抑菌效果，其最小抑菌质量浓度（minimum inhibitory concentration, MIC）为1.6 mg/mL，显示出良好的抑菌活性.
Abstract: Jumi, the flower bud of Chrysanthemum indicum L., is a special tea from Shilian, Zhejiang Province, and it is famous for its unique fragrance. Jumi essential oil has numerous efficacies and applications in the field of pharmacy and cosmetic industry, therefore developing effective oil extracting method which can keep both biological activity and fragrance is of considerable significance. At present, steam distillation has been reported as essential oil extract method, but this method can not keep the unique fragrance of Jumi due to the decomposition of fragrant component at high temperature. However, the supercritical fluid of CO2 (SFECO2) is a low temperature processing technique and can fully keep the unique fragrance of Jumi, which is an efficient, nontoxic, pollutionfree, and nonresidual method to extract and separate Jumi essential oil. The objective of this paper is to evaluate the quality of Jumi essential oil extracted by SFECO2and determine its biological activity. The chemical compositions of Jumi essential oil were determined by GCMS and their inhibitory activities against Staphylococcus aureus and Escherichia coli were analyzed by filter paper tablet bacteriostatic method and minimum inhibitory concentration (MIC). The results showed that the 28 types of components with the similarity of more than 70% were identified in Jumi essential oil by GCMS analysis, which were mainly terpenes and their derivatives, and the former mainly include monoterpene and sesquiterpene, and the latter was mainly alcohols, for example, the contents of hexamethylbenzene, 2(2, 4hexadiynylidene)1,6dioxaspiro［4, 4］ nonane3ene and (2RCis)1, 2, 3, 4, 4a, 5, 6, 7octahydro.alpha., .alpha., 4a, 8tetramethyl2naphthalenemethanol were higher than 9%, respectively. Bacterial inhibition tests indicated that Jumi essential oil could inhibit the growth of S. aureus and E. coli, and its MIC was 1.6 mg/mL. It is indicated that the Jumi essential oil extracted by SFECO2 is nontoxic and pollutionfree and it offers a scientific evidence about developing Jumi essential oil with high additional value and goodquality in cosmetic and food additive.
出版日期: 2013-03-20
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王春荣
方程吉
余庆青
蒋鹏
田薇*

引用本文:

王春荣， 方程吉， 余庆青， 蒋鹏， 田薇*. 菊米超临界CO2萃取物的气相色谱质谱分析及其抑菌活性[J]. 浙江大学学报(农业与生命科学版), 2013, 39(2): 167-172.

WANG Chunrong, FANG Chengji, YU Qingqing, JIANG Peng, TIAN Wei*. Gas chromatography and mass spectrometry (GCMS) analysis of supercritical CO2 extract of flower bud of Chrysanthemum indicum and its antibacterial activity. Journal of Zhejiang University. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(2): 167-172.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2012.11.068    或    http://www.zjujournals.com/agr/CN/Y2013/V39/I2/167

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