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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2012, Vol. 38 Issue (6): 709-714    DOI: 10.3785/j.issn.1008-9209.2012.04.233
农业科学     
喷施6-糠氨基嘌呤对铝胁迫大麦幼苗的缓解效应
郭天荣*， 潘伟槐
绍兴文理学院 生命科学学院，浙江 绍兴 312000
Alleviating effect of 6-kinetin spraying on aluminum toxicity in barley seedlings
GUO Tian-rong*, PAN Wei-huai  
College of Life Sciences, Shaoxing University, Shaoxing, Zhejiang 312000, China
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摘要：   采用营养液培养试验，研究叶面喷施不同浓度6-糠氨基嘌呤 (6-KT) (100、200、400和800 μmol/L) 对铝胁迫大麦幼苗生长、叶片叶绿素含量、游离脯氨酸含量、活性氧清除酶活性的影响. 结果表明：与单独铝胁迫相比，喷施100~400 μmol/L 6-KT 可改善大麦幼苗生长，提高叶绿素含量，降低叶片丙二醛 (malondialdehyde, MDA) 和脯氨酸含量，降低叶片过氧化物酶 (peroxidase, POD) 和超氧化物歧化酶 (superoxide dismutase, SOD) 活性；喷施200和400 μmol/L 6-KT亦可降低过氧化氢酶 (catalase, CAT) 活性；当喷施200 μmol/L 6-KT时各指标的增幅最大，与对照组值最接近，但当喷施浓度为800 μmol/L时植株生物量、叶绿素含量下降，而MDA、脯氨酸含量和抗氧化酶活性上升. 表明适宜浓度的外源6-KT可有效缓解大麦由于铝毒害引发的生长抑制和氧化胁迫，但浓度过高则产生氧化作用.
Abstract:   Aluminum (Al) is considered as the major factor constraining crop performance when soil pH is lower than 5.5. The most easily recognized symptom of A1 toxicity is the inhibition of root growth, and this has become a widely accepted measure of A1 stress in plants. In simple nutrient solutions, micromolar concentrations of A1 can begin to inhibit root growth within 60 min. Kinetin (KT), one of the cytokins, is a unique plant hormone that promotes cell division. KT has been widely used to alleviate the toxicityin plants caused by abiotic stresses, for example, cadmium, lead, and salt stress. However, little attention has been paid to study the alleviating effects of KT on Al toxicity to plants, especially for barley, a major winter crop in South China. In the present study, hydroponic experiments were 6-kinetin (6-KT) on barley seedlings under Al stress by investigating the growth, chlorophyll and proline contents, as well as the activities of antioxidant enzymes in the leaves. conducted to focus on the alleviative effect of spraying Uniform barley seedlings were selected 10 days after sowing and transplanted onto a 5-L pot containing 4.5 L nutrient solution, which was covered with a polystyrol-plate with seven evenly spaced holes, and placed in a greenhouse with the photoperiod of 8 h light/16 h dark and the temperature of (21 ± 2) °C throughout the experiment. One week after transplanting to the basic solution culture, Al as AlCl3·6H2O was added to the nutrient solution. Meanwhile, different concentrations (100, 200, 400, and 800 μmol/L) of 6-KT were sprayed on barley leaves exposed to Al stress solution every other day. No Al and 6-KT but deionized water was sprayed on the leaves growing in control solution to following six treatments. The nutrient solution in the growth container was continuously aerated with pumps and replaced with fresh solution every five days. The experimental results showed that, spraying 100-400 μmol/L 6-KT under Al stress improved plant growth (Table 1), increased chlorophyll contents (Fig. 1), but decreased the contents of free proline (Fig. 4) and malondialdehyde (MDA) (Fig. 2), and decreased the activities of superoxide dismutase (SOD), peroxidase (POD) (Fig.3). Compared with spraying 100 μmol/L 6-KT, the catalase (CAT) activity decreased when the spraying concentrations were 200 and 400 μmol/L (Fig. 3). Furthermore, the optimal alleviation effect was at a concentration of 200 μmol/L 6-KT. At this spraying concentration, the chlorophyll content was increased by 27.5 %, while the MDA content was decreased by 29.5 %, compared with the Al individual treatment. However, when spraying concentration of 6-KT was 800 μmol/L, the plant growth was decreased, while the proline content and antioxidase activities were increased, in addition, the chlorophyll contents were decreased by 4.5 %, and MDA contents were increased by 3.0 %, as compared to the Al individual treatment. Moreover, the experiment demonstrated that free proline content accumulated in barley plants was a toxic symptom but not a resistance mechanism to Al stress. In conclusion, appropriate concentration of exogenous 6-KT can alleviate growth inhibition and oxidative stress caused by Al toxicity in barley seedlings
出版日期: 2012-11-20
基金资助:

浙江省自然科学基金资助项目 (Y3100450)；浙江省教育厅资助项目 (Z201018672).
通讯作者: 郭天荣，Tel: +86-575-88345864; E-mail: guotr@163.com   
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郭天荣*
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引用本文:

郭天荣*， 潘伟槐. 喷施6-糠氨基嘌呤对铝胁迫大麦幼苗的缓解效应[J]. 浙江大学学报(农业与生命科学版), 2012, 38(6): 709-714.

GUO Tian-rong*,PAN Wei-huai. Alleviating effect of 6-kinetin spraying on aluminum toxicity in barley seedlings. , 2012, 38(6): 709-714.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2012.04.233    或    http://www.zjujournals.com/agr/CN/Y2012/V38/I6/709

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