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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2012, Vol. 38 Issue (4): 519-523    DOI: 10.3785/j.issn.1008-9209.2012.04.022
食品科学     
粒毛盘菌多糖脱蛋白方法及其条件优化
孙柘1， 邱涛1， 袁如月1， 钱梅双1， 肖厚荣2， 叶明1
1.合肥工业大学 生物与食品工程学院，安徽 合肥 230009；2.合肥学院 生物与环境工程系，安徽 合肥 230022
Deproteinization method and optimal condition of crude polysaccharide from Lachnum sp
SUN Zhe1, QIU Tao1, YUAN Ruyue1, QIAN Meishuang1, XIAO Hourong2, YE Ming1
1.College of Biotechnology and Food Engineering, HeFei University of Technology, Hefei 230009, China；2. Department of Biological and Environmental Engineering, Hefei University, Hefei 230022, China
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摘要： 分别采用Sevage法、三氯乙酸正丁醇（TCA-NBA）法、酶Sevage法以及酶TCA-NBA法对粒毛盘菌YM281胞外多糖进行脱蛋白。结果表明：用酶TCA-NBA法对粒毛盘菌YM281胞外多糖脱蛋白的效果最佳，蛋白脱除率为51.57%，且此时多糖损失率（3.46%）为最低；进一步采用均匀设计优化该方法的脱蛋白条件，所得最优脱蛋白条件为：粒毛盘菌YM281多糖溶液20 mL、木瓜蛋白酶溶液11 mL、温度42.2 ℃、pH 4、酶解时间3.5 h；除去变性蛋白后，再利用TCA-NBA法脱蛋白2次即可；采用所得的最优方法和条件脱蛋白，其蛋白脱除率高达72.3%，多糖损失率仅为2.93%。
Abstract: Sevage method, TCA-NBA (trichloroacetic acidNbutyl alcohol) method, enzymeSevage method and enzymeTCA-NBA method were used to remove protein from extracellular polysaccharide of Lachnum  YM281, respectively. The results showed that the method of enzymeTCA-NBA was the most effective, by which the proteinremoval rate and the polysaccharideloss rate were 51.57% and 3.46%, respectively. Then the uniform design was employed to optimize the conditions of this method. As a result, the optimal conditions of the  enzymeTCA-NBA method were as follows: polysaccharide solution of Lachnum YM281 of 20 mL, papain solution of 11 mL, pH 4, zymolysis time of 3.5 h at 42.2 ℃; after removing the denatured protein, TCA-NBA method was used remove protein twice. Under this optimal method and conditions, the proteinremoval rate reached up to 72.3% and the polysaccharideloss rate was only 2.93%．
出版日期: 2012-07-25
CLC:  TQ929.2  
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孙柘1
邱涛1
袁如月1
钱梅双1
肖厚荣2
叶明1

引用本文:

孙柘1， 邱涛1， 袁如月1， 钱梅双1， 肖厚荣2， 叶明1. 粒毛盘菌多糖脱蛋白方法及其条件优化[J]. 浙江大学学报(农业与生命科学版), 2012, 38(4): 519-523.

SUN Zhe1, QIU Tao1, YUAN Ruyue1, QIAN Meishuang1, XIAO Hourong2, YE Ming1. Deproteinization method and optimal condition of crude polysaccharide from Lachnum sp. , 2012, 38(4): 519-523.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2012.04.022    或    http://www.zjujournals.com/agr/CN/Y2012/V38/I4/519

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