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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2012, Vol. 38 Issue (1): 48-54    DOI: 10.3785/j.issn.1008-9209.2012.01.007
生物科学与技术     
肺炎链球菌感染的猕猴肺及气管内IFN-γ蛋白与mRNA表达
罗启慧１ ,２ , 章瑶１ , 陈恋１ , 陈正礼１ ,２ , 曾文１ ,２ , 程安春１ ,２ ,３
１ .四川农业大学动物疫病与人类健康四川省重点实验室,四川雅安６２５０１４ ;２ .四川农业大学实验动物工程技术中心/
国家实验用猕猴种源基地,四川雅安６２５０１４ ;３ .四川农业大学预防兽医研究所,四川成都６１１１３０
Expression of IFN‐γ protein and mRNA in lung and trachea of macaque infected with Streptococcus pneumoniae .
LUO Qi‐hui１ ,２ ,ZHANG Yao１ ,CHEN Lian１ ,CHEN Zheng‐li１ ,２ ,ZENG Wen１ ,２ ,CHENG An‐chun１ ,２ ,３
1 . Key L aboratory o f A nimal Disease and H uman Health o f Sichuan Prov ince , Sichuan Agricultural University , Y a′an , Sichuan 625014 , China ; 2 . Provenance Source Depot o f M acaque for National Ex periment / Ex perimental A nimal Engineering Center , Sichuan A gricultural University ,Ya′an , Sichuan 625014 , China ; 3 . Preventive Veterinary Research Institute , Sichuan A gricultural University , Chengdu 611130 , China
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摘要： 采用常规苏木精‐伊红(hematoxylin‐eosim , HE)染色、免疫组织化学和组织原位杂交技术,观察感染肺炎链球菌的猕猴肺及气管组织的病理学变化与γ 干扰素(interferon‐γ , IFN‐γ)在其中的蛋白与mRNA 表达.结果表明:在光镜下感染组的肺呈现大面积充血、出血,淋巴细胞浸润及肺泡腔内出现浆液性渗出物;IFN‐γ 蛋白表达面积均高于健康组( P ＜ ０.０５ ) ;IFN‐γ 蛋白产物在肺血管内的光密度值极显著高于健康组( P ＜ ０.０１ ) ,在气管和肺的其他部位光密度值显著高于健康组( P ＜ ０.０５ ) ;IFN‐γmRNA 在肺气管上皮层、肌层内阳性产物的光密度值均极显著高于健康组( P＜ ０.０１ ) ,肺泡隔、血管内、气管固有层内阳性产物的光密度值均显著高于健康组( P ＜ ０.０５ ) .说明肺炎链球菌感染的猕猴肺出现了明显的红色肝变期病理变化,且肺及气管中IFN‐γ 蛋白及mRNA 表达显著增加,表明IFN‐γ 在肺炎链球菌感染猕猴肺及气管的免疫调节中起到了重要作用.
Abstract: To investigate the expression and distribution of interferon‐γ ( IFN‐γ) in lung and trachea of macaques infected with Streptococcus pneumoniae , the histopathological changes of macaques?lung and trachea were studied by using hematoxylin‐eosin ( HE) stain , where the expression of IFN‐γ was researched by immunohistochemistry and in situ hybridization . The results showed that a large area of lung was hyperemic and hemorrhagic , and there was lymphocytic infiltration in lung and some serous
exudate in alveolar septum . The area of IFN‐γ protein expression in infected macaques was higher than healthy group ( P ＜ ０.０５) .The optical density of IFN‐γ protein in pulmonary intravascular space was significantly higher than that of healthy group ( P ＜０.０１) , and the optical density in other tissues was higher than that of healthy group ( P ＜０.０５ ) . The optical densities of IFN‐γ mRNA in lung , tracheal epithelium and muscular layer were significantly higher than those of healthy group ( P ＜ ０.０１) , while in alveolar septum , lumen of blood vessel and lamina propria of tracheal were higher than those of healthy group ( P ＜０.０５ ) . As a result , the lung presented significant red hepatization in infected macaques , and the expression of IFN‐γ protein and mRNA in lung and trachea increased significantly ,
indicating that IFN‐γ might play an important role in immunoloregulation of macaques?lung and trachea infected by S . pneumoniae .
出版日期: 2012-01-20
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罗启慧
章瑶
陈恋
陈正礼
曾文
程安春

引用本文:

罗启慧,章瑶,陈恋,陈正礼,曾文,程安春. 肺炎链球菌感染的猕猴肺及气管内IFN-γ蛋白与mRNA表达[J]. 浙江大学学报(农业与生命科学版), 2012, 38(1): 48-54.

LUO Qi hui，ZHANG Yao，CHEN Lian，CHEN Zheng li，ZENG Wen，CHENG An chun. Expression of IFN‐γ protein and mRNA in lung and trachea of macaque infected with Streptococcus pneumoniae .. , 2012, 38(1): 48-54.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2012.01.007    或    http://www.zjujournals.com/agr/CN/Y2012/V38/I1/48

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