Please wait a minute...
浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2012, Vol. 38 Issue (1): 28-34    DOI: 10.3785/j.issn.1008-9209.2012.01.004
生物科学与技术     
水稻纹枯病菌菌核形成过程中特异基因的表达与分析
陈晓耀, 王伟, 王政逸, 陈卫良
浙江大学生物技术研究所,浙江杭州３１００５８
Analysis of differentially expressed genes of Rhizoctonia solani during the sclertium formation
CHEN Xiao‐yao , WANG Wei , WANG Zheng‐yi , CHEN Wei‐liang
Institute o f Biotechnology ,Zhe jiang University , Hang zhou 310058 , China
 全文: PDF(4796 KB)   HTML ( ) 
摘要： 　以水稻纹枯病GD‐１１８ 菌株菌丝形成期的mRNA 为驱赶子(driver) ,以菌核形成期的mRNA 为
检测子(tester) ,采用抑制消减杂交(SSH)结合虚拟Northern 杂交筛选,构建了水稻纹枯病菌菌核形成
过程中特异表达产生的差减cDNA 文库;进一步用PCR 筛选显示克隆中插入的片段主要分布在２００ ～
５００ bp 之间;随机挑取克隆应用虚拟Northern 技术以菌丝、菌核cDNA 为探针来验证消减有效性,经测
序和同源性检测剔除重复序列之后,获得了从菌丝到菌核形成相关的２８ 条特异表达的基因片段;所筛
选的特异表达序列主要涉及胁迫响应、转录调控、信号传导、合成代谢等.这为进一步分析水稻纹枯病菌
菌核形成过程的相关基因提供了科学线索.
Abstract: 　Subtractive cDNA library of differentially expressed genes during the sclertium formation of
Rhizoctonia solani , GD‐１１８ strain , was constructed by using suppression subtractive hybridization
(SSH) technique combined with virtual Northern blot . The hypha mRNA was used as the driver and the
sclertium mRNA was applied as the tester . Further PCR screening showed that the size of the inserts
was mainly between ２００ to ５００ bp . The effectiveness of the subtractive hybridization was verified by
virtual Northern blot of colonies randomly selected using hypha , sclertium cDNA sequence as probes .
Finally , ２８ specific sclertium formation‐related gene fragments were obtained after sequencing and
homology‐blasting , which were mainly related to stress response , transcription regulation , signal
transduction and metabolism in the pathogen . Results above provide scientific clues to understand the
mechanism of the sclertium formation of R . solani .
出版日期: 2012-01-20
服务　　
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章　　
陈晓耀
王伟
王政逸
陈卫良

引用本文:

陈晓耀,王伟,王政逸,陈卫良. 水稻纹枯病菌菌核形成过程中特异基因的表达与分析[J]. 浙江大学学报(农业与生命科学版), 2012, 38(1): 28-34.

CHEN Xiao yao,WANG Wei,WANG Zheng yi,CHEN Wei liang. Analysis of differentially expressed genes of Rhizoctonia solani during the sclertium formation. , 2012, 38(1): 28-34.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2012.01.004    或    http://www.zjujournals.com/agr/CN/Y2012/V38/I1/28

No related articles found!