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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (5): 521-526    DOI: 10.3785/j.issn.1008-9209.2011.05.008
生物科学与技术     
甘肃棘豆中降解苦马豆素内生细菌的筛选与鉴定
胡延春１ ,２ , 刘鹏１ , 赵春蕊１ , 郑传廷１ , 刘玲１
１.四川农业大学动物医学院临床兽医学系,四川雅安６２５０１４;２. 四川农业大学动物疫病与人类健康四川省重点实验室,四川雅安６２５０１４
Screening and identification of endophytic bacteria of Oxytropis kansuensis Bunge for swainsonine degradation .
HU Yan‐chun１ ,２, LIU Peng１, ZHAO Chun‐rui１, ZHENG Chuan‐ting１, LIU Ling１

1 . Department of Clinical Veterinary Medicine , College of Veterinary Medicine , Sichuan A gricultural University , Y a＇an , Sichuan 625014 , China ; 2 . Key L aboratory of Animal Disease and Human Health of Sichuan Province , Sichuan A gricultural University , Y a＇an , Sichuan 625014 , China
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摘要： 为研究甘肃棘豆( Oxytropis kansuensis Bunge)中降解疯草毒素——— 苦马豆素的内生细菌,采用选择性富集培养的方法,以苦马豆素作为唯一碳源,从甘肃棘豆内生细菌中筛选到１株苦马豆素降解菌,命名为Ab .在摇床转速为１５０r.min－ １ 、２８ ℃ 条件下培养３０h ,菌株Ab 对质量浓度为１００ mg.L － １苦马豆素的降解率为１１.５７７ １％ ;延长培养时间并不能提高其降解苦马豆素的能力,但可耐受高质量浓度(１ g.L －１)的苦马豆素.通过形态学、生理生化试验和１６S rDNA 序列比对分析对Ab 进行鉴定,结果表明,菌株Ab 为革兰阴性短小杆菌,在LB 平板上菌落呈圆形,中心凸起,边缘整齐,乳白色,表面光滑,属于短波单胞杆菌属( Brevundimonas) ,与缺陷短波单胞菌( Brevundimonas diminuta)同源性为１００％ .说明甘肃棘豆中存在降解苦马豆素的内生细菌Brevundimonas diminuta Ab ;降解苦马豆素的内生细菌可能与产生苦马豆素的内生真菌共同维持疯草中苦马豆素的动态平衡.
Abstract: One strain , named as Ab , which was isolated from Oxytropis kansuensis Bunge and could degrade locoweed toxin — swainsonine , was screened by selective enrichment culture when swainsonine was used as a sole carbon source . Its degradation rate could reach １１ .５７７ １％ under the circumstance of being incubated on a shaker at １５０r.min－１and temperature at ２８℃ within ３０hours when the concentration of swainsonine was １００mg.L － １ . The capability of degrading swainsonine could not be improved by extending cultivation time , while it could tolerate high concentration swainsonine up to １ g.L － １ . Strain Ab was identified by １６S rDNA sequencing , morphological , biochemical and physiological experiments . The results showed that strain Ab was gram‐negative bacteria whose colonies were round ,milk white ,glossy and had raised‐center and regular edge on the Luria‐Bertani plate . And the colony was Brevundimonas and shared the highest homology ( １００％ ) with Brevundimonas diminuta by Blast software analysis . It is concluded that one strain of endophytic bacterium for degrading swainsonine , B .diminuta strain Ab , was existed in O . kansuensis . Therefore ,it is speculated that endophytic bacteria of degrading swainsonine might keep the homeostasis of swainsonine together with endophytic fungi of producing swainsonine in locoweeds .
出版日期: 2011-09-20
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胡延春
刘鹏
赵春蕊
郑传廷
刘玲

引用本文:

胡延春, 刘鹏, 赵春蕊, 郑传廷, 刘玲. 甘肃棘豆中降解苦马豆素内生细菌的筛选与鉴定[J]. 浙江大学学报(农业与生命科学版), 2011, 37(5): 521-526.

HU Yan‐chun,LIU Peng, ZHAO Chun‐rui, ZHENG Chuan‐ting, LIU Ling. Screening and identification of endophytic bacteria of Oxytropis kansuensis Bunge for swainsonine degradation .. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(5): 521-526.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.05.008    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I5/521

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