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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (5): 509-515    DOI: 10.3785/j.issn.1008-9209.2011.05.006
生物科学与技术     
蝴蝶兰叶片对低温胁迫的生理响应
高冬冬, 谭艳玲, 马关喜, 周伟军, 黄冲平
浙江大学农业与生物技术学院作物科学研究所,浙江杭州３１００２９
Physiological responses of Phalaenopsis leaves to low temperature stress .
GAO Dong‐dong , TAN Yan‐ling , MA Guan‐xi , ZHOU Wei‐jun , HUANG Chong‐ping
Institute of Crop Science , College o f A griculture & Biotechnology , Zhejiang University , Hangzhou 310029 , China.
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摘要： 以３个蝴蝶兰品种(系) (耐冷品种'婚宴',不耐冷品种'聚宝',中等耐冷性新品系'ZD‐１')为试验材料,经预处理后,测定各材料在低温(１０ ℃ 、５ ℃ 和０ ℃ )下处理１２ 、２４ 和４８h 后叶片相对电导率、叶片中可溶性糖和丙二醛(MDA)含量以及过氧化氢酶(CAT) 、超氧化物歧化酶(SOD)与抗坏血酸过氧化物酶(APX)的活性.结果表明:随着胁迫程度的加深,蝴蝶兰叶片的相对电导率和MDA 含量逐渐增加,可作为蝴蝶兰耐冷性的鉴定指标;可溶性糖含量的变化无明显规律;CAT 和SOD 酶活性随着胁迫程度的加深先增大后减小;APX 活性则呈持续增加趋势,也可作为蝴蝶兰耐冷性的鉴定指标;耐冷性品种'婚宴'在一定程度低温胁迫条件下能通过自身酶系统的保护,减少活性氧对其的伤害,而不耐冷品种
聚宝'的自身酶系统的保护能力较差.
Abstract: In order to study the physiological characteristics of three typical Phalaenopsis spp . varieties (cold‐tolerant variety 'Hunyan' cold‐susceptible variety 'ubao', medium cold‐tolerant variety 'ZD‐１') under low temperature stress , the activities of catalase (CAT) , superoxide dismutase (SOD) and ascorbate peroxidase (APX) , relative electrical conductivity , soluble sugar and malondialdehyde (MDA) contents in leaves of Phalaenopsis spp . were determined at １０℃ , ５℃ , ０℃ for １２, ２４, ４８h after pretreatment. The results showed that the relative electrical conductivity and MDA contents in leaves were enhanced along with the increase of low temperature stress . Therefore , these two physiological indexes could be used as identification indexes of cold tolerance for Phalaenopsis plant . No obvious changes of soluble sugar contents were detected . The activities of CAT and SOD increased from １２h to ２４h and decreased from ２４h to ４８h at all low temperature treatments . The activity of APX consistently increased with the increase of stress degree . The activity of APX could also be used as an identification index of cold tolerance .It was found that the variety ?Hunyan?with good tolerance to low temperature possessed self‐protection mechanism through enzyme defense systems , while the susceptible variety 'Jubao'had low protection ability to cold stress .
出版日期: 2011-09-20
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高冬冬
谭艳玲
马关喜
周伟军
黄冲平

引用本文:

高冬冬, 谭艳玲, 马关喜, 周伟军, 黄冲平. 蝴蝶兰叶片对低温胁迫的生理响应[J]. 浙江大学学报(农业与生命科学版), 2011, 37(5): 509-515.

GAO Dong‐dong,TAN Yan‐ling,MA Guan‐xi,ZHOU Wei‐jun,HUANG Chong‐ping.. Physiological responses of Phalaenopsis leaves to low temperature stress .. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(5): 509-515.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.05.006    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I5/509

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