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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (5): 479-484    DOI: 10.3785/j.issn.1008-9209.2011.05.002
生物科学与技术     
豌豆EST‐SSR 标记在蚕豆中的通用性与应用
龚亚明１, 徐盛春１, 毛伟华２, 李泽昀２
1.浙江省农业科学院蔬菜研究所,浙江杭州３１００２１;２浙江大学分析测试中心,浙江杭州３１００２９
Transferable analysis of pea EST‐SSRs on faba bean and its application
GONG Ya‐ming１, XU Sheng‐chun１, MAO Wei‐hua２, LI Ze‐yun２
1 Institute of Vegetables , ZhejiangA cademy o f A gricultural Sciences , Hangz hou 310021 , China ; 2 Center of Analysis and Measurement , Zhe jiang University ,Hangzhou 310029 , China
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摘要： 对豌豆表达序列标签‐微卫星(EST‐SSR)分子标记在蚕豆中的通用性与应用进行研究,以期为蚕豆开发出新的分子标记.结果表明:１６３对豌豆EST‐SSR 引物中有９９对可在蚕豆中获得有效扩增,其中３６对引物呈现明显的多态性,共检测到等位位点１４８个,平均每个位点的等位基因变异数为４.１个;各多态性引物的多态信息量(PIC)值变化范围为０.０３５到０.８１０ ,平均PIC 值为为０畅４８３４ ;实际观察杂合度( HO )变化范围为０.０００１到０.７００ ０ ,平均值为０.２１０３ ;期望杂合度( HE )变化范围为０.０３５７到０.８４５２ ,平均值为０.５３８８ ;主成分分析与非加权配对算术平均法(UPGMA)聚类分析表明,３０份蚕豆种质资源可明显分成２大类群.以上结果表明,本研究所开发的豌豆EST‐SSR标记在蚕豆上具有通用性和多态性,可为今后蚕豆遗传多样性、种质资源保存、比较作图和分子标记辅助育种提供新的研究依据.
Abstract: In order to develop novel molecular markers for faba bean ( V icia f aba L.) , the transferability of pea ( Pisum sativum L.) EST‐SSR (expressed sequence tag‐simple sequence repeats) markers and their application were studied in faba bean germplasms.The results revealed that ９９of １６３‐pair primers for pea EST SSRsachieved the effective amplification in faba bean , indicating a significant transferability ( ６０.７４％ ) of pea ESTSSR primers to faba bean.Thirty‐six of these transferable markers exhibited polymorphism and totally １４８alleles were detected with an average variation of ４.１.The polymorphism information contents of polymorphic markers varied from ０.０３５to ０.８１０ with an average of ０.４８３４, while observed heterozygosity ( HO ) ranged from ０.０００１to ０.７０００and expected heterozygosity ( HE ) ranged from ０.０３５７to ０.８４５２,with an average of ０.２１０３and ０.５３８８respectively.Furthermore , UPGMA ( unweight pair group method with arithemetic
averages) analysis and principal coordinate analysis (PCA) revealed that the ３０germplasms were classified into two major clusters.Because of their polymorphism and transferability , the developed markers in this study will provide the valuable tool for genetic diversity , resource conservation , comparative mapping
and marker‐assisted breeding of faba bean in the future.
出版日期: 2011-09-20
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龚亚明
徐盛春
毛伟华
李泽昀

引用本文:

龚亚明, 徐盛春, 毛伟华, 李泽昀. 豌豆EST‐SSR 标记在蚕豆中的通用性与应用[J]. 浙江大学学报(农业与生命科学版), 2011, 37(5): 479-484.

GONG Ya‐ming, XU Sheng‐chun, MAO Wei‐hua, LI Ze‐yun. Transferable analysis of pea EST‐SSRs on faba bean and its application. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(5): 479-484.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.05.002    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I5/479

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