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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (4): 407-412    DOI: 10.3785/j.issn.1008-9209.2011.04.008
生物科学与技术     
利用分子标记辅助选育油菜隐性上位互作核不育临保系
倪西源1，2,徐小栋2,黄吉祥2,陈 飞2,周伟军1,赵坚义2
１ .浙江大学作物科学研究所,浙江杭州３１００５８ ;２ .浙江省农业科学院作物与核技术利用研究所,浙江杭州３１００２１
Marker‐assisted selection for temporary maintainers of a recessive epistemic genic male sterility in Brassica napus L
NI Xi-yuan1，2,XU Xiao-dong2,HUANG Ji-xiang2,CHEN Fei2,ZHOU Wei-jun1,ZHAO Jian-yi2
1 .Institute of Crop Science , Zhejiang University , Hangzhou 310058 , China ; 2 .Institute of Crop and Nuclear Technology Utiliz ation , Zhejiang A cademy of Agricultural Sciences , Hangzhou 310021 ,China
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摘要： 以湘油１５ 、沪油１５ 、优８８ 和沪油１７ 分别与临保系HYZ1-TAM 杂交,在其F２ 利用与不育基因Bnms3 和隐性上位基因Bnr f 连锁的分子标记对临保系基因型单株进行筛选,并对筛选获得的单株进行测交验证确定是否为临保系基因型.结果表明:在４ 个品种的转育F２ 群体共３５５ 个单株中,利用分子标记辅助选择获得了６２ 个标记基因型为临保系的单株,这些单株经测交证实有５７ 株为真实临保系,分子标记辅助选择的准确率为９１.９４％ .根据实验结果推测,本研究所用的４ 个甘蓝型油菜品种在Bnms4基因位点未出现显性可育等位基因.实验结果验证了分子标记辅助选择具有较高的准确率,对开展该不育系统的转育工作具有很好的借鉴意义.
Abstract: Four varieties of Brassica napus , Xiangyou １５ , Huyou １５ , You ８８ and Huyou １７ , were crossed with a temporary maintainer ( TAM ) named HYZ1-TAM respectively and produced four F２ populations . In these populations , molecular markers associated with Bnms3 and Bnr f were used to select new temporary maintainer plants which were identified by test‐cross . Sixty‐two plants with TAM marker‐genotype were selected by marker‐assisted selection ( MAS) from three hundred and fifty‐five plants of four F２ populations . Fifty‐seven plants ( with the accuracy of 91.94% ) were TAM genotype proved by test‐cross . Compared with traditional method , it is very efficient to select TAM by MAS . In the Bnms4 locus , all the four varieties have a same genotype which is ms4ms4 .
出版日期: 2011-07-20
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倪西源
徐小栋
黄吉祥
陈 飞
周伟军
赵坚义

引用本文:

倪西源,徐小栋,黄吉祥,陈 飞,周伟军,赵坚义. 利用分子标记辅助选育油菜隐性上位互作核不育临保系[J]. 浙江大学学报(农业与生命科学版), 2011, 37(4): 407-412.

NI Xi-yuan,XU Xiao-dong,HUANG Ji-xiang,CHEN Fei,ZHOU Wei-jun,ZHAO Jian-yi. Marker‐assisted selection for temporary maintainers of a recessive epistemic genic male sterility in Brassica napus L. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(4): 407-412.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.04.008    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I4/407

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