Please wait a minute...
浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (4): 371-379    DOI: 10.3785/j.issn.1008-9209.2011.04.003
生物科学与技术     
鸭PPARα基因结构及功能的生物信息学分析
马  云1,王云云1,2,张晓婷1,李 芬1,王启钊1,3,王新庄4
１ .信阳师范学院生命科学学院,河南信阳４６４０００ ;２ .华中科技大学生命科学与技术学院,湖北武汉４３００７４ ;
３ .浙江大学原子核农业科学研究所,浙江杭州３１００２９ ;４ .河南农业大学牧医工程学院, 河南郑州４５０００２
Bioinformatics analysis of duck PPARα gene structure and function
MA Yun１ , WANG Yun-yun１ ,２ , ZHANG Xiao-ting１ , LI Fen１ , WANG Qi-zhao１ ,３ ,WANG Xin-zhuang４
1 . College of Life Sciences , Xinyang Normal University , Xinyang , Henan 464000 , China ;2 . School o f L i f e Science and Technology , Huazhong University of Science & Technology ,Wuhan 430074 , China ;3 . Institute of Nuclear Agricultural Science , Zhej iang University , Hangzhou 310029 ,China ;4 . College of Animal Husbandry and Veterinary Medicine ,Henan Agricultural University ,Zhengzhou 450002 ,China
 全文: PDF(7243 KB)   HTML ( ) 
摘要： 以鸭的过氧化物酶体增殖物激活受体α( PPARα)基因为研究对象,运用生物信息学方法对其编码的蛋白质结构、理化性质及功能结构域进行分析.结果表明:鸭的PPA Rα 基因cDNA 全长１ ４３０ bp ,最长开放阅读框为１ ４０７ bp ,编码４６８ 个氨基酸;序列比对结果显示鸭的PPA Rα 基因与鸡、胸草雀、人、牛、家马等该基因的核苷酸序列同源性分别为９６％ 、９２％ 、７９％ 、７７％ 和７８％ ,鸭PPA Rα 基因编码的氨基酸序列与上述其他动物的同源性高达９８％ 、９７％ 、８９％ 、８９％ 和８８％ ;系统进化分析结果显示,在鸡、胸草雀、人、牛、马等动物中,鸭的PPA Rα 基因与鸡的进化关系最为密切,亲缘关系最近;鸭的PPARα 蛋白中α 螺旋较多,该蛋白质含有１ 段短的核定位信号序列KKNRNKC ,在引导该蛋白质进入细胞核的过程中发挥作用,同时还有由２ 个锌指结构组成的DNA 结合域及１ 段配体结合域.
Abstract: Protein structures , physical and chemical properties , and their functional domain structures encoded by duck peroxisome proliferators‐activated receptors‐α ( PPARα ) gene were analyzed using bioinformatics method . Sequencing and bioinformatics analysis showed that the duck PPA Rα cDNA sequence was １ ４３０ base pairs long with an open reading frame ( ORF) containing １ ４０７ nucleotides encoding a protein of ４６８ amino acids . The multiple sequence alignment showed that coding nucleotide sequences of the obtained duck PPA Rα gene had ９６％ , ９２％ , ７９％ , ７７％ , ７８％ homology with the corresponding chicken , T aeniopygia guttata , human , bovine and Equus caballus , respectively , and the deduced amino acid sequences had ９８％ , ９７％ , ８９％ , ８９％ , ８８％ identity with the corresponding species , respectively . Phylogenetic tree analysis of the amino acid sequences of duck PPAR α and other PPARα proteins in human and other animals showed that , among all the species mentioned above , the nearest relationship existed between duck and chicken . Further analysis on the structure and function of the duck PPARα protein showed that there were much more α helix than other secondary structure in the duck PPARα protein , besides , it had a short nuclear localization signal (KKNRNKC) , so that it could enter into nucleus . Moreover , a DNA binding domain which was composed of two C４‐type zinc fingers and a ligand binding domain was found in the PPARα protein .
出版日期: 2011-07-20
服务　　
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章　　
马 云
王云云
张晓婷
李 芬
王启钊
王新庄

引用本文:

马 云,王云云,张晓婷,李 芬,王启钊,王新庄. 鸭PPARα基因结构及功能的生物信息学分析[J]. 浙江大学学报(农业与生命科学版), 2011, 37(4): 371-379.

MA Yun,WANG Yun-yun, ZHANG Xiao-ting, LI Fen, WANG Qi-zhao,WANG Xin-zhuang. Bioinformatics analysis of duck PPARα gene structure and function. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(4): 371-379.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.04.003    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I4/371

No related articles found!