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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (3): 332-337    DOI: 10.3785/j.issn.1008-9209.2011.03.015
食品科学     
均匀设计法优选菱壳中抗胃癌成分的半仿生提取工艺
宁颖1,于海宁2,周新妹3,王维4,蒋风4,潘晓燕3,沈生荣1
１ .浙江大学生物系统工程与食品科学学院,浙江杭州３１００５８ ;２ .浙江工业大学药学院,浙江杭州３１００１４ ;３ .嘉兴学院医学院,浙江嘉兴３１４００１ ;４ .杭州华东医药集团康润制药有限公司,浙江安吉３１３３０１
Optimal process for semibionic extraction of bioactive compounds against gastric cancer from water chestnutshell by uniform  designation
NING Ying1,YU Hai-ning2,ZHOU Xin-me3i,WANG Wei4,JIANG Feng4,PAN Xiao-yan3,SHEN Sheng-rong1
1 . School of Biosystems Engineering and Food Science , Zhejiang University , Hangzhou 310058 , China ; 2 . College of Pharmaceutical Science , Zhejiang University of Technology ,Hangzhou 310014 , China ; 3 . School of Medicine , Jiaxing College , Jiaxing , Zhejiang 314001 ,China ; 4 . Hangzhou Huadong Medicine Group Kangrun Pharmaceutical Co . L td . , A nj i , Zhejiang 313301 , China
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摘要： 以SGC７９０１ 胃癌细胞存活率为指标,运用半仿生提取方法回收菱壳中的抗胃癌活性成分,考察提取时间、料液比、胃蛋白酶用量、胰蛋白酶用量对提取物抗胃癌活性的影响,并运用均匀设计法确定其最佳提取工艺.结果表明:菱壳与pH ≈ ２ 提取液按料液质量体积比１ ∶ １０ ,不加入胃蛋白酶,３７ ℃ 提取３０ min 后,离心收集沉淀,添加４％ 胰蛋白酶,菱壳与pH ≈ ８ 提取液按料液质量体积比１ ∶ １０ ,３７ ℃提取３０ min ,可获得最佳抑制效果,细胞存活率仅为４５.５２ ％ .本实验表明,采用半仿生法提取菱壳中的抗胃癌活性成分是可行的.
Abstract: The viability rate of SGC７９０１ gastric cancer cells was used as an index , the anti‐gastric cancer ingredients from the water chestnut shell were reclaimed by semi‐bionic extraction . The effects of extraction time , ratio of water to water chestnut shell , amount of pepsin and trypsin on extraction antigastric cancer activity were investigated , and the uniform designation was employed to determine the optimum extraction conditions . The results showed that when １ g shell powder was added to １０ mL solvent (１ ∶ １０ ratio of powder to pH ≈ ２ solvent) in the absence of pepsin , extracting for ３０ min at ３７℃ , and then being centrifuged to collect precipitate , adding １０ mL pH ≈ ８ solvent and ４％ trypsin , extracting for ３０ min at ３７ ℃ , the extracts had the highest activity against gastric cancer and the cell
viability was the lowest ,i .e . ４５.５２％ .Also , this experiment confirmed that water chestnut shell extracts still had a high activity of inhibiting the growth of gastric cancer cells through semi‐bionic extraction .
出版日期: 2011-05-20
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宁颖
于海宁
周新妹
王维
蒋风
潘晓燕
沈生荣

引用本文:

宁颖,于海宁,周新妹,王维,蒋风,潘晓燕,沈生荣. 均匀设计法优选菱壳中抗胃癌成分的半仿生提取工艺[J]. 浙江大学学报(农业与生命科学版), 2011, 37(3): 332-337.

NING Ying,YU Hai-ning,ZHOU Xin-mei,WANG Wei,JIANG Feng,PAN Xiao-yan,SHEN Sheng-r. Optimal process for semibionic extraction of bioactive compounds against gastric cancer from water chestnutshell by uniform  designation. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(3): 332-337.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.03.015    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I3/332

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