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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (3): 319-325    DOI: 10.3785/j.issn.1008-9209.2011.03.013
农业科学     
芽孢杆菌在肉鸡肠道内的分布及对肠道菌群、消化酶活性的影响
汤江武,孙宏,姚晓红,吴逸飞,王新
浙江省农业科学院植物保护与微生物研究所, 浙江杭州 ３１００２１
Fate,dissemination of Bacillus subtilis spores in gastrointestinal tract of broiler chicken and its effect onintestinal flora and digestive enzyme activities
TANG Jiang-wu,SUN Hong,YAO Xiao-hong,WU Yi-fei,WANG Xin
Institute of Plant Protection and M icrobiology , Zhejiang Academy of A gricultural Sciences ,Hangzhou 310021 ,China
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摘要： 考察枯草芽孢杆菌的芽孢在AA 肉鸡肠道内的分布和随粪便的排出规律,及对肠道内细菌群落结构变化和肠道内消化酶活性的影响.试验选取２４０ 羽１ 日龄AA 肉鸡, 随机分为２ 组,每组６ 个重复,采用相同的无抗生素基础日粮饲养３ d 后,对照组和试验组分别一次性灌喂无菌水或枯草芽孢杆菌的芽孢(１.５ × １０８ CFU.mL -１ )各０.５ mL ;在不同时间采样进行计数和酶活性测定,同时通过聚合酶链反应-变性梯度凝胶电泳( polymerase chain reaction-denaturing gradient gel electrophoresis , PCRDGGE)技术分析此过程肉鸡粪便中细菌群落的变化.结果表明:灌喂的芽孢在十二指肠内开始萌发,以空肠的营养体数量最高,盲肠内则以芽孢为主;粪便中的芽孢数量在２４ h 时最高,达到７.９ × １０４ CFU.g-１,而后逐渐降低,在７２ h 后基本无法检出.PCR‐DGGE 结合主成分分析(PCA)表明:对照组与试验组鸡粪中的细菌群落结构存在较大差异,其中试验组中肠道有益菌----- 乳杆菌属( L actobacillus sp .)含量较对照组有明显提高;同时,灌喂芽孢１２ h 后,十二指肠内淀粉酶、胰蛋白酶和总蛋白酶活性,空肠内的脂肪酶活性和盲肠内的总蛋白酶活性均高于对照,差异有统计学意义( P ＜ ０.０５ ) .试验证实芽孢能在AA 肉鸡肠道内萌发并停留超过７２ h ,并对提高肠道内消化酶活性和改善菌落结构发挥作用.
Abstract: Two hundred forty one-day-old AA broilers were randomly divided into ２ groups with ６replicates ,administrating the same antibiotics-free basal diet . After ３ d feeding , broilers of control group and experimental group were respectively dosed with ０.５ mL sterile water or spore ( １.５ × １０８ CFU.mL －１ ) , aiming to examine the internal fate and dissemination of Bacillus subtilis spores in the intestine of AA broiler as well as its effects on changes of intestinal flora and activity of digestive enzymes .Spores were counted and enzyme activities were measured at different sampling time , while the intestinal flora was determined by using polymerase chain reaction‐denaturing gradient gel electrophoresis (PCR‐DGGE) method . The results showed that : feeding spores began to germinate in the duodenum ; vegetative cells outnumbered spores in the jejunum whereas most of B . subtilis spores were detected in ceca . Spores in faeces reached its peak value of ７.９ × １０４ CFU.g --１ at ２４ h after dosed , then were gradually decreased .After ７２ h ,no spores could be detected . PCR‐DGGE combined with principal component analysis (PCA)
showed significant changes in the intestinal flora during passage of B . subtilis spores , while Lactobacillus sp . population in experimental group increased , compared with the control group . Amylase , trypsin and total protease activities in duodenum , lipase activity in jejunum and total protease activities in cecum were significantly higher than control group ( P ＜ ０.０５) after １２ h of spores dosing .The results above confirm that spores can survive the gastric barrier well , germinate in the intestine of
AA broiler , and are detected up to ７２ h . In such process , intestinal digestive enzyme activity and microflora are improved .
出版日期: 2011-05-20
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汤江武
孙宏
姚晓红
吴逸飞
王新

引用本文:

汤江武,孙宏,姚晓红,吴逸飞,王新. 芽孢杆菌在肉鸡肠道内的分布及对肠道菌群、消化酶活性的影响[J]. 浙江大学学报(农业与生命科学版), 2011, 37(3): 319-325.

TANG Jiang-wu,SUN Hong,YAO Xiao-hong,WU Yi-fei,WANG Xin. Fate,dissemination of Bacillus subtilis spores in gastrointestinal tract of broiler chicken and its effect onintestinal flora and digestive enzyme activities. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(3): 319-325.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.03.013    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I3/319

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