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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (3): 289-294    DOI: 10.3785/j.issn.1008-9209.2011.03.008
生物科学与技术     
Wnt1和Frizzled 2在性成熟前小鼠卵巢组织定位及配受体关系鉴定
庞训胜1,林春联2,许雪萍1,王正朝3
1 .安徽科技学院动物科学学院, 安徽 凤阳 233100 ;2 .安徽科技学院图书馆,安徽 凤阳 233100 ;3 .弗吉尼亚联邦大学 弗吉尼亚医学院, 美国 里士满 23298
Localization of Wnt1 and Frizzled 2 and identification of their ligand receptor relationship in the ovary tissue of immature mice
PANG Xun-sheng1,LIN Chun-lian2,XU Xue-ping1,WANG Zheng-chao3
1 . College o f A nimal Science , Anhui Science and Technology University , Fengyang , Anhui 233100 , China ; 2 . Library ,Anhui Science and Technology University , Fengyang ,  Anhui 233100 , China ; 3 . Medical College of Virginia , V irginia Commonwealth University , Richmond VA 23298 , USA
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摘要： 为揭示Wnt1 和Frizzled (Fzd) 2 参与卵巢和卵泡发育的作用机制,选择12 只3 周龄小鼠,应用免疫组化研究Wnt1 和Fzd2 在卵巢组织内的定位,免疫共沉淀鉴定其蛋白间的配受体关系.结果表明:在健康初级、次级卵泡卵母细胞以及腔前和腔后卵泡近基膜颗粒细胞的胞质和胞膜中,Wnt1 均表现较强的免疫染色,Fzd2 在胞质内免疫染色;在卵泡腔周围颗粒细胞和透明带周围卵丘细胞中Wnt1 免疫染色弱;从卵巢中心扩展或环绕生长卵泡的条形基质细胞中Wnt1 和Fzd2 免疫染色阳性,间质腺存在免疫染色细胞;在闭锁卵泡卵母细胞中Wnt1 和Fzd2 呈非均匀强染色;在卵母细胞裂解液的Wnt1 或Fzd2 免疫沉淀物的电泳图谱中,分别同时存在Fzd2 和Wnt1 蛋白条带.表明Wnt1 定位于卵母细胞和近基膜颗粒细胞的胞质和胞膜中,Fzd2 定位于胞质内;在卵巢基质细胞、卵泡膜细胞和间质腺细胞中存在Wnt1 和Fzd2 分布;Wnt1 和Fzd2 之间可能为配体-受体关系.
Abstract: In order to reveal the mechanism of Wnt１ and Frizzled (Fzd) ２ in the regulation of ovarian and follicular development , twelve ３‐week old mice were used to investigate the localization of Wnt1 and Fzd2 in the ovary by immunohistochemistry (IHC) and their ligand‐receptor relationship by coimmunoprecipitation (Co-IP) . The results showed that a strong immunostaining of Wnt1 was found in the cytoplasm and on the member of oocytes of health primary and secondary follicles , as well as that in
the granular cells near basal lamina of preantral and antral follicles , however Fzd2 staining was found only in the cytoplasm . There was weakly immunostaining of Wnt1 in granular cells around antrum and cumulus oophorus around zona pellucida . The elongated stromal cells round developing follicles or branched from ovarian cores were immunostained by Wnt1 and Fzd2 . Furthermore , some scattered cells were also immunostained in the interstitial gland , while in the oocytes of follicular atresia , a strong and asymmetrical immunostaining was also found . Co-IP analysis showed that there was a direct interaction between Fzd2 and Wnt1 in the ovarian oocytes . Together , the results over clearly demonstrated the expression and localization of Wnt1 and Fzd2 in a cell-specific manner and their ligand-receptor relation , implying the important roles during ovarian follicular development .
出版日期: 2011-05-20
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庞训胜
林春联
许雪萍
王正朝

引用本文:

庞训胜,林春联,许雪萍,王正朝. Wnt1和Frizzled 2在性成熟前小鼠卵巢组织定位及配受体关系鉴定[J]. 浙江大学学报(农业与生命科学版), 2011, 37(3): 289-294.

PANG Xun-sheng,LIN Chun-lian,XU Xue-ping,WANG Zheng-chao. Localization of Wnt1 and Frizzled 2 and identification of their ligand receptor relationship in the ovary tissue of immature mice. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(3): 289-294.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.03.008    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I3/289

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