Please wait a minute...

Current Issue

, Volume 6 Issue 6   
Differentiation of xanthomonads causing the bacterial leaf spot of poinsettia in China from the pathotype strain of Xanthomonas axonopodis pv. poinsettiicola*
LI Bin, XIE Guan-lin, SWINGS J.
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 1-.   https://doi.org/10.1631/jzus.2005.B0451
Abstract   PDF (0KB)
In October 2003, a new bacterial disease with symptoms similar to those caused by Xanthomonas axonopodis pv. poinsettiicola was observed on poinsettia leaves at a flower nursery in Zhejiang Province of China. Three Xanthomonas strains were isolated from infected plants and classified as X. axonopodis. They were differentiated from the pathotype strain LMG849 of X. axonopodis pv. poinsettiicola causing bacterial leaf spot of poinsettia by comparison of pathogenicity, substrate utilization and BOX-PCR genomic fingerprints.
Spatio-temporal expression of the pathway−specific regulatory gene redD in S. coelicolor*
ZHOU Li-hua, LI Yu-qin, LI Yong-quan, WU Dan
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 4-.   https://doi.org/10.1631/jzus.2005.B0464
Abstract   PDF (0KB)
Confocal laser scanning microscopy was used to observe the spatio-temporal expression of the pathway-specific gene redD during S. coelicolor cell cultivation. The corresponding mutant S. coelicolor lyqRY1522 carrying redD::eyfp in the chromosome
Maximal sequence length of exact match between members from a gene family during early evolution*
WEN Xiao, GUO Xing-yi, FAN Long-jiang
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 5-.   https://doi.org/10.1631/jzus.2005.B0470
Abstract   PDF (0KB)
Mutation (substitution, deletion, insertion, etc.) in nucleotide acid causes the maximal sequence lengths of exact match (MALE) between paralogous members from a duplicate event to become shorter during evolution. In this work, MALE changes betw
Identification of Rhodiola species by using RP-HPLC*
WANG Qiang, RUAN Xiao, JIN Zhi-hua, YAN Qi-chuan, TU Shanjun
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 6-.   https://doi.org/10.1631/jzus.2005.B0477
Abstract   PDF (0KB)
An approach was established using RP-HPLC (reversed-phase high-performance liquid chromatography) to identify ten species of Rhodiola, R. coccinea A. Bor, R. junggarica C.Y. Yang et N.R. Cui spn., R. heterodonta A. Bor, R. linearifolia A. Bor, R
Firmness evaluation of melon using its vibration characteristic and finite element analysis*
NOURAIN Jamal, YING Yi-bin, WANG Jian-ping, RAO Xiu-qin, YU Chao-gang
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 7-.   https://doi.org/10.1631/jzus.2005.B0483
Abstract   PDF (0KB)
The ?°Huang gua?± melons were measured for their physical properties including firmness and static elastic modulus. The vibrational characteristics of fruits and vegetables are governed by their elastic modulus (firmness), mass, and geometry. Ther
Suppression of Meloidogyne javanica by antagonistic and plant growth-promoting rhizobacteria*
LI Bin, XIE Guan-lin, SOAD A., COOSEMANS J.
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 9-.   https://doi.org/10.1631/jzus.2005.B0496
Abstract   PDF (0KB)
Four rhizobacteria selected out of over 500 isolates from rhizosphere of the vegetables in China were further studied for suppression of the root-knot nematode and soil-borne fungal pathogens in laboratory and greenhouse in Belgium. They were id
Characteristics of fruit ripening in tomato mutant epi*
WANG Zhong-feng, YING Tie-jin, BAO Bi-li, HUANG Xiao-dan
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 10-.   https://doi.org/10.1631/jzus.2005.B0502
Abstract   PDF (0KB)
The characteristics of fruit ripening and expression of ripening-related genes were investigated in epi, an ethylene overproduction mutant of tomato (Lycopersicon esculentum Mill.). The epi produces apparently more ethylene than its wild type VF
Optimization of angiotensin I-converting enzyme (ACE) inhibition by rice dregs hydrolysates using response surface methodology
HE Guo-qing, XUAN Guo-dong, RUAN Hui, CHEN Qi-he, XU Ying
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 11-.   https://doi.org/10.1631/jzus.2005.B0508
Abstract   PDF (0KB)
Angiotensin I-converting enzyme (ACE) inhibitory peptides have been shown to have antihypertensive effects and have been utilized for physiologically functional foods and pharmaceuticals. The ACE inhibitory ability of a hydrolysate is determined by its peptide composition. However, the peptide composition of a hydrolysate depends on proteolytic enzyme and the hydrolysis conditions. In this study, the effect of process conditions on the ACE inhibitory activity of rice dregs hydrolyzed with a trypsin was investigated systematically using response surface methodology. It was shown that the ACE inhibitory activity of rice dregs hydrolysates could be controlled by regulation of five process conditions. Hydrolysis conditions for optimal ACE inhibition were defined using the response surface model of fractional factorial design (FFD), steepest ascent design, and central composite design (CCD).
Application of derivative ratio spectrophotometry for determination of |?-carotene and astaxanthin from Phaffia rhodozyma extract*
NI Hui, HE Guo-qing, RUAN Hui, CHEN Qi-he, CHEN Feng
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 12-.   https://doi.org/10.1631/jzus.2005.B0514
Abstract   PDF (0KB)
A derivative ratio spectrophotometric method was used for the simultaneous determination of |?-carotene and astaxanthin produced from Phaffia rhodozyma. Absorbencies of a series of the standard carotenoids in the range of 441 nm to 490 nm demonst
Prediction and analysis model of temperature and its application to a natural ventilation multi-span plastic greenhouse equipped with insect-proof screen*
LIU Shu-zhen, HE Yong, ZHANG Yu-bao, MIAO Xiang-wen
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 13-.   https://doi.org/10.1631/jzus.2005.B0523
Abstract   PDF (0KB)
The natural ventilation widely used in greenhouses has advantages of saving energy and reducing expense. In order to provide information for climate control of greenhouse, a model was developed to predict the variation of air temperature in the
On-line estimation of concentration parameters in fermentation processes*
XIONG Zhi-hua, HUANG Guo-hong, SHAO Hui-he
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 14-.   https://doi.org/10.1631/jzus.2005.B0530
Abstract   PDF (0KB)
It has long been thought that bioprocess, with their inherent measurement difficulties and complex dynamics, posed almost insurmountable problems to engineers. A novel software sensor is proposed to make more effective use of those measurements
Automatic separation system for marine meiobenthos based on laser-induced fluorescence technology*
YE Shu-ming, GAO Shao-jia, PAN Yi, YANG Jun-yi, LI Shi-lun
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 15-.   https://doi.org/10.1631/jzus.2005.B0535
Abstract   PDF (0KB)
An automatic system for marine meiobenthos separation was developed by using laser-induced fluorescence technology. Rose Bengal was used as organism dye and the spectrums of Rose Bengal were measured. Laser-induced fluorescence system was established to detect marine meiobenthos in sediments. Data obtained from experiments were analyzed by using a mathematical model. The results showed that laser-induced fluorescence technology worked well in the system. The system could select the meiobenthos efficiently and precisely.
Remediation of Cr(VI) in solution using vitamin C*
LIU Yong, XU Xin-hua, HE Ping
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 16-.   https://doi.org/10.1631/jzus.2005.B0540
Abstract   PDF (0KB)
The effectiveness of vitamin C in treating Cr(VI)-contaminated water is being evaluated. Cr(VI) is an identified pollutant of some soils and groundwater. Vitamin C, an important biological reductant in humans and animals, can be used to transform Cr(VI) to essentially nontoxic Cr(III). The removal efficiency was 89% when the mass concentration of vitamin C was 80 mg/L in 60 min, and nearly 100% Cr(VI) was removed when the mass concentration was 100 mg/L. Our data demonstrated that the removal efficiency was affected by vitamin C concentration, the reaction temperature and the dissolved oxygen concentration. The reaction mechanism of Cr(VI) by vitamin C was presented. Our study opens the way to use vitamin C to remediate Cr(VI)-contaminated soils and groundwater.
A new configuration of membrane stack for retrieval of nickel absorbed in resins*
CHEN Xue-fen, WU Zu-cheng
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 17-.   https://doi.org/10.1631/jzus.2005.B0543
Abstract   PDF (0KB)
A new configuration integrated ion exchange effect system with both electro-migration and electrochemical reaction in a single cell was developed to effectively retrieve metal ions from simulated wastewater using ion exchange resins without additive chemicals. By simply assembling cation exchange resins and anion exchange resins separated by homogeneous membranes, we found that the system will always be acidic in the concentrate compartment so that ion exchange resins could be in-situ regenerated without hydroxide precipitation. Such a realizable design will be really suitable for wastewater purification.
Changes of root morphology and Pb uptake by two species of Elsholtzia under Pb toxicity*
PENG Hong-yun, TIAN Sheng-ke, YANG Xiao-e
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 18-.   https://doi.org/10.1631/jzus.2005.B0546
Abstract   PDF (0KB)
Elsholtzia argyi and Elsholtzia splendens, which are Chinese endemic Pb/Zn mined and Cu mined ecotype respectively, were investigated in the aspect of their response to Pb toxicity in the presence or absence of EDTA addition. After 8 d?ˉs Pb trea
Study on US/O3 mechanism in p-chlorophenol decomposition
XU Xian-wen, XU Xin-hua, SHI Hui-xiang, WANG Da-hui
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 19-.   https://doi.org/10.1631/jzus.2005.B0553
Abstract   PDF (0KB)
Study on the effects of sonolysis, ozonolysis and US/O3 system on the decomposition of p-chlorophenol in aqueous solutions indicated that in the cases of US/O3 system, individual ozonolysis and sonolysis, the decomposition rate of p-chlorophenol reached 78.78%, 56.20%, 2.79% after a 16-min reaction while its CODcr (chemical oxygen demand) removal rate was 97.02%, 62.17%, 3.67% after a 120-min reaction. The decomposition reaction of p-chlorophenol follows pseudo-first-order kinetics. The enhancement factors of p-chlorophenol and its CODcr under US/O3 system reached 63% and 237% respectively. The main intermediates during the decomposition include catechol, hydroquinone, p-benzoquinone, phenol, fumaric acid, maleic acid, oxalic acid and formic acid. The decomposition mechanism of p-chlorophenol was also discussed.
Study on anaerobic treatment of wastewater containing hexavalent chromium*
XU Yan-bin, XIAO Hua-hua, SUN Shui-yu
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 23-.   https://doi.org/10.1631/jzus.2005.B0574
Abstract   PDF (0KB)
A self-made anaerobic bio-filter bed which was inoculated with special sludge showed high efficiency in removing hexavalent chromium. When pump flow was 47 ml/min and CODCr of wastewater was about 140 mg/L, it took 4 h to decrease the Cr6+ concentrations from about 60 mg/L to under 0.5 mg/L, compared with 14 h without carbon source addition. Cr6+ concentrations ranged from 64.66 mg/L to 75.53 mg/L, the system efficiency was excellent. When Cr6+ concentration reached 95.47 mg/L, the treatment time was prolonged to 7.5 h. Compared with the contrast system, the system with trace metals showed clear superiority in that the Cr6+ removal rate increased by 21.26%. Some analyses also showed that hexavalent chromium could probably be bio-reduced to trivalent chromium, and that as a result, the chrome hydroxide sediment was formed on the surface of microorganisms.
Preparation of high-permeability NiCuZn ferrite*
HU Jun, YAN Mi
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 24-.   https://doi.org/10.1631/jzus.2005.B0580
Abstract   PDF (0KB)
Appropriate addition of CuO/V2O5 and the reduction of the granularity of the raw materials particle decrease the sintering temperature of NiZn ferrite from 1200 ??C to 930 ??C. Furthermore, the magnetic properties of the NiZn ferrite prepared at low temperature of 930 ??C is superior to that of the NiZn ferrite prepared by sintering at high temperature of 1200 ??C because the microstructure of the NiZn ferrite sintered at 930 ??C is more uniform and compact than that of the NiZn ferrite sintered at 1200 ??C. The high permeability of 1700 and relative loss coefficient tan|?/|ìi of 9.0?á10−6 at 100 kHz was achieved in the (Ni0.17Zn0.63Cu0.20)Fe1.915O4 ferrite.
Study on the prediction of visible absorption maxima of azobenzene compounds
LIU Jun-na, CHEN Zhi-rong, YUAN Shen-feng
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 25-.   https://doi.org/10.1631/jzus.2005.B0584
Abstract   PDF (0KB)
The geometries of azobenzene compounds are optimized with B3LYP/6-311G* method, and analyzed with nature bond orbital, then their visible absorption maxima are calculated with TD-DFT method and ZINDO/S method respectively. The results agree well
Interaction of nonionic surfactant AEO9 with ionic surfactants*
ZHANG Zhi-guo, YIN Hong
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 27-.   https://doi.org/10.1631/jzus.2005.B0597
Abstract   PDF (0KB)
The interaction in two mixtures of a nonionic surfactant AEO9 (C12H25O(CH2CH2O)9H) and different ionic surfactants was investigated. The two mixtures were AEO9/sodium dodecyl sulfate (SDS) and AEO9/cetyltrimethylammonium bromide (CTAB) at molar
Stability and electronic spectra of C76N2 isomers
TENG Qi-wen, WU Shi
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 28-.   https://doi.org/10.1631/jzus.2005.B0602
Abstract   PDF (0KB)
Study of geometries of 16 possible isomers for C76N2 based on C78(C2v) by intermediate neglect of differential overlap (INDO) series of methods indicated that the most stable geometry 25,78-C76N2 where two nitrogen atoms substitute two apexes C(25) and C(78) near the shortest X axis and Y axis formed by two hexagons and a pentagon. Electronic structures and spectra of C76N2 were investigated. The reason for the red-shift for absorptions of C76N2 compared with that of C78(C2v) is discussed.
Quantum chemical study on asymmetric allylation of benzaldehyde in the presence of chiral allylboronate
CHEN Wan-suo, CHEN Zhi-rong
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 29-.   https://doi.org/10.1631/jzus.2005.B0606
Abstract   PDF (0KB)
The quantum chemical method is employed to study the modified asymmetric allylation of benzaldehyde controlled by diisopropyl D-(-)-tartrate auxiliary. All the structures are optimized completely at the B3LYP/6-31G(d,p) level. The (R)-secondary alcohol can be achieved mainly through a six-membered ring chair-like transition state structure. From the relative reaction rates theory the main product configuration predicted is in agreement with the experiment result.
22 articles

NoticeMore

Links