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| Endophytes from medicinal plants and their potential for producing indole acetic acid, improving seed germination and mitigating oxidative stress |
| Abdul Latif Khan, Syed Abdullah Gilani, Muhammad Waqas, Khadija Al-Hosni, Salima Al-Khiziri, Yoon-Ha Kim, Liaqat Ali, Sang-Mo Kang, Sajjad Asaf, Raheem Shahzad, Javid Hussain, In-Jung Lee, Ahmed Al-Harrasi |
| UoN Chair of Oman's Medicinal Plants & Marine Natural Products, University of Nizwa, Nizwa 616, Oman; Department of Biological Sciences & Chemistry, University of Nizwa, Nizwa 616, Oman; School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Korea; Department of Agriculture, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan; Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA |
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Abstract Medicinal plants have been used by marginal communities to treat various ailments. However, the potential of endophytes within these bio-prospective medicinal plants remains unknown. The present study elucidates the endophytic diversity of medicinal plants (Caralluma acutangula, Rhazya stricta, and Moringa peregrina) and the endophyte role in seed growth and oxidative stress. Various organs of medicinal plants yielded ten endophytes, which were identified as Phoma sp. (6 isolates), Alternaria sp. (2), Bipolaris sp. (1), and Cladosporium sp. (1) based on 18S rDNA sequencing and phylogenetic analysis. The culture filtrates (CFs; 25%, 50%, and 100% concentrations) from these endophytes were tested against the growth of normal and dwarf mutant rice lines. Endophytic CF exhibited dose-dependent growth stimulation and suppression effects. CF (100%) of Phoma sp. significantly increased rice seed germination and growth compared to controls and other endophytes. This growth-promoting effect was due to the presence of indole acetic acid in endophytic CF. The gas chromatography/mass spectrometry (GC/MS) analysis showed the highest indole acetic acid content ((54.31±0.21) µmol/L) in Bipolaris sp. In addition, the isolate of Bipolaris sp. exhibited significantly higher radical scavenging and anti-lipid peroxidation activity than the other isolates. Bipolaris sp. and Phoma sp. also exhibited significantly higher flavonoid and phenolic contents. The medicinal plants exhibited the presence of bio-prospective endophytic strains, which could be used for the improvement of crop growth and the mitigation of oxidative stresses.
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Received: 28 January 2015
Published: 26 January 2017
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Cite this article:
Abdul Latif Khan, Syed Abdullah Gilani, Muhammad Waqas, Khadija Al-Hosni, Salima Al-Khiziri, Yoon-Ha Kim, Liaqat Ali, Sang-Mo Kang, Sajjad Asaf, Raheem Shahzad, Javid Hussain, In-Jung Lee, Ahmed Al-Harrasi. Endophytes from medicinal plants and their potential for producing indole acetic acid, improving seed germination and mitigating oxidative stress. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2017, 18(2): 125-137.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1500271 OR http://www.zjujournals.com/xueshu/zjus-b/Y2017/V18/I2/125
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