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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2014, Vol. 15 Issue (11): 997-1005    DOI: 10.1631/jzus.B1400051
Articles     
Development of 107 SSR markers from whole genome shotgun sequences of Chinese bayberry (Myrica rubra) and their application in seedling identification
Hui-min Jia, Yu-tong Shen, Yun Jiao, Guo-yun Wang, Xiao Dong, Hui-juan Jia, Fang Du, Sen-miao Liang, Chao-chao Zhou, Wei-hua Mao, Zhong-shan Gao
Department of Horticulture, Zhejiang University, Hangzhou 310058, China; Fruit Research Institute, Yuyao 315400, China; Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; Yuyao Agricultural and Forestry Bureau, Yuyao 315400, China; Bio-Macromolecules Analysis Lab, Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310058, China
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Abstract  Chinese bayberry (Myrica rubra Sieb. et Zucc.) is one of the important subtropical fruit crops native to the South of China and Asian countries. In this study, 107 novel simple sequence repeat (SSR) molecular markers, a powerful tool for genetic diversity studies, cultivar identification, and linkage map construction, were developed and characterized from whole genome shotgun sequences. M13 tailing for forward primers was applied as a simple method in different situations. In total, 828 alleles across 45 accessions were detected, with an average of 8 alleles per locus. The number of effective alleles ranged from 1.22 to 10.41 with an average of 4.08. The polymorphic information content (PIC) varied from 0.13 to 0.89, with an average of 0.63. Moreover, these markers could also be amplified in their related species Myrica cerifera (syn. Morella cerifera) and Myrica adenophora. Seventy-eight SSR markers can be used to produce a genetic map of a cross between 鈥楤iqi鈥?and 鈥楧ongkui鈥? A neighbor-joining (NJ) tree was constructed to assess the genetic relationships among accessions, and the elite accessions 鈥榊2010-70鈥? 鈥榊2012-140鈥? and 鈥榊2012-145鈥? were characterized as potential new genotypes for cultivation.

Key words： Chinese bayberry      Simple sequence repeat (SSR)      Table S1

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Received: 21 February 2014      Published: 04 November 2014
CLC:  S667.6  
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Articles by authors
Chao-chao Zhou
Wei-hua Mao
Zhong-shan Gao
Hui-min Jia
Yu-tong Shen
Yun Jiao
Guo-yun Wang
Xiao Dong
Hui-juan Jia
Fang Du
Sen-miao Liang
Cite this article:

Hui-min Jia, Yu-tong Shen, Yun Jiao, Guo-yun Wang, Xiao Dong, Hui-juan Jia, Fang Du, Sen-miao Liang, Chao-chao Zhou, Wei-hua Mao, Zhong-shan Gao. Development of 107 SSR markers from whole genome shotgun sequences of Chinese bayberry (Myrica rubra) and their application in seedling identification. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(11): 997-1005.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1400051     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I11/997

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