| Biotechnology |
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| Moisture sorption characteristics of freeze-dried human platelets |
| Meng-jie Xu, Guang-ming Chen, Ju-li Fan, Jin-hui Liu, Xian-guo Xu, Shao-zhi Zhang |
| Institute of Refrigeration and Cryogenics, Zhejiang University, Hangzhou 310027, China, College of Aerospace Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China, Key Laboratory of Blood Safety Research, Ministry of Health, Blood Center of Zhejiang Province, Hangzhou 310006, China |
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Abstract Freeze-drying is a promising method for a long-term storage of human platelets. The moisture sorption characteristics of freeze-dried human platelets (FDHPs) were studied in this paper. The moisture sorption isotherms of FDHPs and freeze-dried lyophilization buffer (FDLB) were measured at 4, 25, and 37 °C. The experimental data were fitted to Brunauer-Emmett-Teller (BET) and Guggenheim-Anderson-de Boer (GAB) equations. There were no significant statistical differences (P>0.05) between the sorption characteristics of FDHPs and FDLB at 4 and 25 °C, while FDHPs absorbed more water at 37 °C. The net isosteric heat of sorption was derived. The heat for FDHPs showed an abnormal negative value at low moisture contents when 25 and 37 °C data were used. Dynamic sorption experiments were carried out at 25 °C with environmental water activity controlled at 0.75, 0.85, and 0.90. The moisture diffusion coefficient was fitted to be 8.24×10−12 m2/s when experimental data at initial time were used. These results would be helpful in choosing prehydration and storage condition for FDHPs.
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Received: 23 April 2010
Published: 09 March 2011
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