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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2009, Vol. 10 Issue (5): 368-374    DOI: 10.1631/jzus.B0820285
Biomedicine     
Adiponectin levels are associated with the number and activity of circulating endothelial progenitor cells in patients with coronary artery disease
Zhi-qiang YING, Dan-dan ZHONG, Geng XU, Miao-yan CHEN, Qing-yu CHEN
Department of Cardiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; Department of Internal Medicine, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China
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Abstract  Objective: To study the relationship between plasma adiponectin concentration and the functional activities of circulating endothelial progenitor cells (EPCs) in patients with coronary artery disease (CAD). Methods: Circulating EPCs were enumerated as AC133+/KDR+ cells via flow cytometry and identified by co-staining with DiI-acLDL and fluorescein isothiocyanate (FITC)-conjugated lectin under a fluorescent microscope. The migratory capacity of EPCs was measured by modified Boyden chamber assay. Adhesion capacity was performed to count adherent cells after replating EPCs on six-well culture dishes coated with fibronectin. Results: The number of circulating EPCs (AC133+/KDR+ cells) decreased significantly in CAD patients, compared with control subjects [(74.2±12.3) vs (83.5±12.9) cells/ml blood, P<0.01]. In addition, the number of EPCs also decreased in CAD patients after ex vivo cultivation [(54.4±8.6) vs (71.9±11.6) EPCs/field, P<0.01]. Both circulating EPCs and differentiated EPCs were positively correlated with plasma adiponectin concentration. The functional activities of EPCs from CAD patients, such as migratory and adherent capacities, were also impaired, compared with control subjects, and positively correlated with plasma adiponectin concentration. Conclusion: The study demonstrates that the impairment of the number and functional activities of EPCs in CAD patients is correlated with their lower plasma adiponectin concentrations.

Key words： Adiponectin      Adipokines      Endothelial progenitor cells (EPCs)      Coronary artery disease (CAD)     
Received: 15 September 2008     
CLC:  R54  
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Zhi-qiang YING
Dan-dan ZHONG
Geng XU
Miao-yan CHEN
Qing-yu CHEN
Cite this article:

Zhi-qiang YING, Dan-dan ZHONG, Geng XU, Miao-yan CHEN, Qing-yu CHEN. Adiponectin levels are associated with the number and activity of circulating endothelial progenitor cells in patients with coronary artery disease. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(5): 368-374.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0820285     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2009/V10/I5/368

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