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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2009, Vol. 10 Issue (2): 133-141    DOI: 10.1631/jzus.B0820183
Biotechnology     
ISSR markers based on GA and AG repeats reveal genetic relationship among rice varieties tolerant to drought, flood, or salinity
Ch Surendhar REDDY, A. Prasad BABU, B.P. Mallikarjuna SWAMY, K. KALADHAR, N. SARLA
Biotechnology Laboratory, Crop Improvement Section, Directorate of Rice Research, Rajendranagar, Hyderabad 500030, India
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Abstract  Drought, flood, salinity, or a combination of these limits rice production. Several rice varieties are well known for their tolerance to specific abiotic stresses. We determined genetic relationship among 12 rice varieties including 9 tolerant to drought, flood, or salinity using inter-simple sequence repeat (ISSR) markers. Based on all markers, the nine tolerant varieties formed one cluster distinct from the cluster of three control varieties. The salt-tolerant varieties were closest to two flood-tolerant varieties, and together they were distinct from the drought-tolerant varieties. (GA)8YG was the most informative primer, showing the highest polymorphic information content (PIC) and resolving power (Rp). The drought-, flood-, and salt-tolerant varieties grouped in three distinct clusters within the group of tolerant varieties, when (GA)8YG was used. Sabita was the only exception. The two aus varieties, Nagina22 and FR13A, were separated and grouped with the drought- and flood-tolerant varieties, respectively, but they were together in dendrograms based on other primers. The results show that ISSR markers associated with (GA)8YG delineated the three groups of stress-tolerant varieties from each other and can be used to identify genes/new alleles associated with the three abiotic stresses in rice germplasm.

Key words： Drought      Submergence      Salinity      Inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR)      (GA)8YG      Nagina22 (N22)      FR13A      Pokkali     
Received: 04 June 2008     
CLC:  Q78  
  S51  
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Ch Surendhar REDDY
A. Prasad BABU
B.P. Mallikarjuna SWAMY
K. KALADHAR
N. SARLA
Cite this article:

Ch Surendhar REDDY, A. Prasad BABU, B.P. Mallikarjuna SWAMY, K. KALADHAR, N. SARLA. ISSR markers based on GA and AG repeats reveal genetic relationship among rice varieties tolerant to drought, flood, or salinity. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(2): 133-141.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0820183     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2009/V10/I2/133

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