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Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2009, Vol. 10 Issue (11): 847-851    DOI: 10.1631/jzus.B0920098
Biotechnology     
Differential expression of salt tolerance related genes in Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee
Yang QIU, Xi-xiang LI, Hai-ying ZHI, Di SHEN, Peng LU
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
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Abstract  We examined salt tolerance responsive genes in Pak-choi under salt stress and analyze their potential function. The mRNA differential display was used to screen the transcript derived fragments (TDFs) related to salinity tolerance in tolerant and moderately tolerant Pak-choi germplasm. Seventy-eight primer combinations generated 101 differential cDNA fragments, which were divided into 10 expression types. Seven cDNA sequences (GenBank accession Nos. DQ006915~DQ006921) obtained and sequenced were highly homologous to some known expression genes or the genes related to the signaling pathways in plants under different abiotic stress.

Key wordsBrassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee      Salinity tolerance      Gene differential expression      cDNA fragments      Basic local alignment search tool (BLAST) analysis     
Received: 09 April 2009     
CLC:  S63  
  Q37  
Cite this article:

Yang QIU, Xi-xiang LI, Hai-ying ZHI, Di SHEN, Peng LU. Differential expression of salt tolerance related genes in Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(11): 847-851.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0920098     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2009/V10/I11/847

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