| Biomedicine |
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| T-2 toxin-induced apoptosis involving Fas, p53, Bcl-xL, Bcl-2, Bax and caspase-3 signaling pathways in human chondrocytes |
| Jing-hong CHEN, Jun-ling CAO, Yong-lie CHU, Zhi-lun WANG, Zhan-tian YANG, Hong-lin WANG |
| Ministry of Education Key Laboratory of Environment and Genes related to Diseases, Institute of Endemic Diseases, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China |
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Abstract Objective: To investigate the effects of T-2 toxin on expressions of Fas, p53, Bcl-xL, Bcl-2, Bax and caspase-3 on human chondrocytes. Methods: Human chondrocytes were treated with T-2 toxin (1~20 ng/ml) for 5 d. Fas, p53 and other apoptosis-related proteins such as Bax, Bcl-2, Bcl-xL, caspase-3 were determined by Western blot analysis and their mRNA expressions were determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Increases in Fas, p53 and the pro-apoptotic factor Bax protein and mRNA expressions and a decrease of the anti-apoptotic factor Bcl-xL were observed in a dose-dependent manner after exposures to 1~20 ng/ml T-2 toxin, while the expression of the anti-apoptotic factor Bcl-2 was unchanged. Meanwhile, T-2 toxin could also up-regulate the expressions of both pro-caspase-3 and caspase-3 in a dose-dependent manner. Conclusion: These data suggest a possible underlying molecular mechanism for T-2 toxin to induce the apoptosis signaling pathway in human chondrocytes by regulation of apoptosis-related proteins.
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Received: 11 January 2008
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Cite this article:
Jing-hong CHEN, Jun-ling CAO, Yong-lie CHU, Zhi-lun WANG, Zhan-tian YANG, Hong-lin WANG. T-2 toxin-induced apoptosis involving Fas, p53, Bcl-xL, Bcl-2, Bax and caspase-3 signaling pathways in human chondrocytes. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2008, 9(6): 455-463.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0820013 OR http://www.zjujournals.com/xueshu/zjus-b/Y2008/V9/I6/455
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