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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2007, Vol. 8 Issue (2): 88-97    DOI: 10.1631/jzus.2007.B0088
Biotechnology     
Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea
JIN Qing-chao, DONG Hai-tao, PENG You-liang, CHEN Bao-shan, SHAO Jing, DENG Ye, DAI Cheng-en, FANG Yong-qi, LOU Yi-chun, LI You-zhi, LI De-bao
Bioinformatics and Gene Network Research Group, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; Key Laboratory of Molecular Plant Pathology, Ministry of Agriculture, China Agricultural University, Beijing 100094, China; Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning 530004, China
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Abstract  Appressorium is an infection structure of the phytopathogenic fungus Magnaporthe grisea. Analysis of gene expression profiles of appressorium development provides insight into the molecular basis of pathogenicity and control of this fungal plant disease. A cDNA array representing 2927 unique genes based on a large EST (expressed sequence tag) database of M. grisea strain Y34 was constructed and used to profile the gene expression patterns at mycelium and appressorium maturation stages. Compared with mycelia, 55 up-regulated and 22 down-regulated genes were identified in mature appressoria. Among 77 genes, 16 genes showed no similarity to the genome sequences of M. grisea. A novel homologue of peptidyl-prolyl cis-trans isomerase was found to be expressed at low-level in mature appressoria of M. grisea. The results indicated that the genes such as pyruvate carboxylase, phospholipid metabolism-related protein and glyceraldehyde 3-phosphate dehydrogenase involved in gluconeogenesis, lipid metabolism and glycolysis, showed differential expression in mature appressoria. Furthermore, genes such as PTH11, beta subunit of G protein and SGT1 involved in cell signalling, were expressed differentially in mature appressoria. Northern blot analysis was used to confirm the cDNA array results.

Key words： Magnaporthe grisea      Mature appressoria      cDNA array      Gene expression profile     
Received: 10 April 2006     
CLC:  Q949.32  
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FANG Yong-qi
LOU Yi-chun
LI You-zhi
LI De-bao
JIN Qing-chao
DONG Hai-tao
PENG You-liang
CHEN Bao-shan
SHAO Jing
DENG Ye
DAI Cheng-en
Cite this article:

JIN Qing-chao, DONG Hai-tao, PENG You-liang, CHEN Bao-shan, SHAO Jing, DENG Ye, DAI Cheng-en, FANG Yong-qi, LOU Yi-chun, LI You-zhi, LI De-bao. Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2007, 8(2): 88-97.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2007.B0088     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2007/V8/I2/88

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