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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2007, Vol. 8 Issue (9): 661-665    DOI: 10.1631/jzus.2007.B0661
Biotechnology     
Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea
LU Jian-ping, DUAN Zhi-bing, LIU Tong-bao, LIN Fu-cheng
Department of Biology, School of Life Sciences, Zhejiang University, Hangzhou 310058, China; Biotechnology Institute, Zhejiang University, Hangzhou 310029, China
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Abstract  The promoter of NAR gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the “TATA” box, the “CAAT” box, and binding sites for fungal regulatory proteins. Programs that predict promoter sequences indicated that promoter sequence lies between locations 430 and 857 of the NAR promoter fragment. GFP expression under the NAR promoter and NAR transcript analysis revealed that this promoter is activated primarily at the mycelial stage in the rice blast fungus and could be used to express native or extrinsic genes in the mycelia of the rice blast fungus.

Key words： Magnaporthe grisea      Promoter      NAR      Clone      Hypha     
Received: 08 May 2007     
CLC:  S432  
  Q933  
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LU Jian-ping
DUAN Zhi-bing
LIU Tong-bao
LIN Fu-cheng
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LU Jian-ping, DUAN Zhi-bing, LIU Tong-bao, LIN Fu-cheng. Cloning, sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2007, 8(9): 661-665.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2007.B0661     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2007/V8/I9/661

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