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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2005, Vol. 6 Issue (11): 3-    DOI: 10.1631/jzus.2005.B1063
    
Dependence of the E. coli promoter strength and physical parameters upon the nucleotide sequence
BEREZHNOY Andrey Y., SHCKORBATOV Yuriy G.
National Scientific Center, Kharkov Physical-Technical Institute, Kharkov 61108, Ukraine; Institute of Biology, Kharkov National University, Kharkov 61077, Ukraine
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Abstract  The energy of interaction between complementary nucleotides in promoter sequences of E. coli was calculated and visualized. The graphic method for presentation of energy properties of promoter sequences was elaborated on. Data obtained indicated that energy distribution through the length of promoter sequence results in picture with minima at –35, –8 and +7 regions corresponding to areas with elevated AT (adenine-thymine) content. The most important difference from the random sequences area is related to –8. Four promoter groups and their energy properties were revealed. The promoters with minimal and maximal energy of interaction between complementary nucleotides have low strengths, the strongest promoters correspond to promoter clusters characterized by intermediate energy values.

Key words： DNA sequence      Promoter strength      Nucleotide pair energy      −35 sequence      −10 sequence      +7 sequence     
Received: 07 July 2005     
CLC:  Q78  
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BEREZHNOY Andrey Y.
SHCKORBATOV Yuriy G.
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BEREZHNOY Andrey Y., SHCKORBATOV Yuriy G.. Dependence of the E. coli promoter strength and physical parameters upon the nucleotide sequence. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(11): 3-.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2005.B1063     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2005/V6/I11/3

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