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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2006, Vol. 7 Issue (1 ): 5-    DOI: 10.1631/jzus.2006.B0028
    
Promoter trapping in Magnaporthe grisea
Liu Xiao-hong, Lu Jian-ping, Wang Jiao-yu, Min Hang, Lin Fu-cheng
Biotechnology Institute, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; School of Life Sciences, Zhejiang University, Hangzhou 310029, China
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Abstract  Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures of M. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis.

Key words： Promoter trapping      Green fluorescent protein      Magnaporthe grisea     
Received: 18 August 2005     
CLC:  Q949.32  
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Liu Xiao-hong
Lu Jian-ping
Wang Jiao-yu
Min Hang
Lin Fu-cheng
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Liu Xiao-hong, Lu Jian-ping, Wang Jiao-yu, Min Hang, Lin Fu-cheng. Promoter trapping in Magnaporthe grisea. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(1 ): 5-.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2006.B0028     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2006/V7/I1 /5

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