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| Genome-wide analysis of OCT4 binding sites in glioblastoma cancer cells |
| Xue-feng Fang, Wei-yi Zhang, Na Zhao, Wei Yu, Dong Ding, Xu Hong, Li-sha Li, Hua-rong Zhang, Shu Zheng, Biao-yang Lin |
| Key Laboratory of Cancer Prevention and Intervention of Ministry of Education, Key Laboratory of Molecular Biology in Medical Sciences of Zhejiang Province, Cancer Institute, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China, Systems Biology Division, Zhejiang-California International Nanosystems Institute (ZCNI), Zhejiang University, Hangzhou 310029, China |
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Abstract OCT4, a member of the POU family of gene products, is an octamer motif-binding transcription factor. As it is known to play a crucial role in cancer processes including proliferation, invasion, and chemoradioresistance, it is important to identify the direct targets of OCT4 in living cancer cells. Here, chromatin immunoprecipitation-sequencing (ChIP-seq) was used to identify OCT4 binding sites in glioblastoma cancer cells. The results showed that 5438 OCT4 binding sites were localized in the glioblastoma cancer genome and that these sites contained a consensus sequence TTTkswTw (k=T or G, s=C or G, w=A or T), which occurred 3931 times in 2312 OCT4 binding regions. Furthermore, binding motifs of some other transcription factors were identified in OCT4 binding regions. Our results provide a valuable dataset for understanding gene regulation mechanisms underlying the function of OCT4 in glioblastoma cancer.
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Received: 01 March 2011
Published: 08 October 2011
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Cite this article:
Xue-feng Fang, Wei-yi Zhang, Na Zhao, Wei Yu, Dong Ding, Xu Hong, Li-sha Li, Hua-rong Zhang, Shu Zheng, Biao-yang Lin. Genome-wide analysis of OCT4 binding sites in glioblastoma cancer cells. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2011, 12(10): 812-819.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1100059 OR http://www.zjujournals.com/xueshu/zjus-b/Y2011/V12/I10/812
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