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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2009, Vol. 10 Issue (8): 619-624    DOI: 10.1631/jzus.B0920153
Biomedicine     
Protective paracrine effect of mesenchymal stem cells on cardiomyocytes
Mei-xiang XIANG, Ai-na HE, Jian-an WANG, Chun GUI
Department of Cardiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China
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Abstract  Objective: The aim of this study was to test the protective effect of mesenchymal stem cells (MSCs) on cardiomyocytes in vitro and to investigate the anti-apoptotic signaling pathway. Methods: MSCs from Sprague-Dawley (SD) rats were separated and cultured. MSC medium was collected from MSCs cultured in serum-free Dulbecco’s modified eagle medium (DMEM) under hypoxia. Cultured cardiomyocytes from neonatal SD rats were exposed to hypoxia/reoxygenation (H/R) and treated with MSC medium. The apoptotic cardiomyocytes were stained with Annexin-V-fluorescein isothiocyanate (FITC), Hoechst 33342 and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). The mitochondrial transmembrane potential of cardiomyocytes was assessed using a fluorescence microscope. The expression of Bcl-2, Bax, cytochrome C, apoptosis-induced factor (AIF), and caspase-3 was tested by Western blot analysis. Results: Our data demonstrated that MSC medium reduced H/R-induced cardiomyocyte apoptosis, increased the Bcl-2/Bax ratio, and reduced the release of cytochrome C and AIF from mitochondria into the cytosol. Conclusion: MSCs protected the cardiomyocytes from H/R-induced apoptosis through a mitochondrial pathway in a paracrine manner.

Key words： Mesenchymal stem cell (MSC)      Apoptosis      Mitochondrial transmembrane potential      Hypoxia/reoxygenation (H/R)     
Received: 25 May 2009     
CLC:  R54  
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Mei-xiang XIANG
Ai-na HE
Jian-an WANG
Chun GUI
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Mei-xiang XIANG, Ai-na HE, Jian-an WANG, Chun GUI. Protective paracrine effect of mesenchymal stem cells on cardiomyocytes. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(8): 619-624.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0920153     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2009/V10/I8/619

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