| Biomedicine |
|
|
|
|
| Quantitative profiles of the mRNAs of ER-α and its novel variant ER-α36 in breast cancers and matched normal tissues |
| Yi ZHENG, Jing ZHANG, Zhen-zhen XU,Jian-ming SHENG, Xiao-chen ZHANG, Hao-hao WANG, Xiao-dong TENG, Xiao-jiao LIU, Jiang CAO, Li-song TENG |
| Cancer Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; Key Laboratory of Biotherapy of Zhejiang Province, Sir Run Run Shaw Institute of Clinical Medicine, Zhejiang University, Hangzhou 310016, China; Department of Pathology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; Department of General Surgery, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China |
|
|
|
Abstract Objective: The novel estrogen receptor-α (ER-α) variant ER-α36 is reported to be functional in the estrogen signaling pathway and is related to tamoxifen resistance in breast cancer. However, ER-α36 tends to be a favorable factor for survival in patients without tamoxifen therapy. To investigate the mechanisms behind this paradox, we determined the differences between the transcriptional profiles of ER-α36 and full-length ER-α (ER-α66) in breast cancers and matched normal tissues. Methods: We analyzed ER-α36 and ER-α66 messenger RNA (mRNA) levels in 74 pairs of breast cancers and matched normal tissues using a real-time quantitative polymerase chain reaction (PCR) assay, and correlated the results with their clinicopathological characteristics. Results: Breast cancers expressed lower ER-α36 mRNA levels than matched normal tissues regardless of their ER-α66 expression status. Down-regulation of ER-α36 mRNA was correlated with local progression, lymph node metastasis, and advanced cancer stage. The level of ER-α66 mRNA was lower in ER-α negative breast cancers compared with matched normal tissues. No differences in ER-α66 mRNA levels were observed during cancer progression. Conclusion: Down-regulation of ER-α36 is associated with carcinogenesis and progression of breast cancer.
|
|
Received: 30 August 2009
|
|
|
|
Cite this article:
Yi ZHENG, Jing ZHANG, Zhen-zhen XU,Jian-ming SHENG, Xiao-chen ZHANG, Hao-hao WANG, Xiao-dong TENG, Xiao-jiao LIU, Jiang CAO, Li-song TENG. Quantitative profiles of the mRNAs of ER-α and its novel variant ER-α36 in breast cancers and matched normal tissues. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(2): 144-150.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0900266 OR http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I2/144
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
