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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2008, Vol. 9 Issue (5): 351-355    DOI: 10.1631/jzus.B0710596
Plant Sciences     
Transient expression of chicken alpha interferon gene in lettuce
Li SONG, De-gang ZHAO, Yong-jun WU, Yi LI
Guizhou Key Laboratory of Ago-Bioengineering, Guizhou University, Guiyang 550025, China; Ministry of Education Key Laboratory of Green Pesticide and Ago-Bioengineering, Guizhou University, Guiyang 550025, China; College of Life Sciences, Guizhou University, Guiyang 550025, China; Department of Plant Science, University of Connecticut, Storrs, CT 06269, USA
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Abstract  We investigated the possibility of producing chicken alpha interferon (ChIFN-α) in transgenic plants. The cDNA encoding ChIFN-α was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system. The ChIFN-α gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF). The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.

Key words： Chicken alpha interferon (ChIFN-α)      Expression      Transgenic lettuce      Bioactivity     
Received: 20 November 2007     
CLC:  TQ464  
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Yong-jun WU
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De-gang ZHAO
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Li SONG, De-gang ZHAO, Yong-jun WU, Yi LI. Transient expression of chicken alpha interferon gene in lettuce. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2008, 9(5): 351-355.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0710596     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2008/V9/I5/351

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