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| Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells |
| Xin-ping Liang, Dong-qiang Zhang, Yan-yan Chen, Rui Guo, Jie Wang, Cheng-zhang Wang, Ying-hua Shi |
| College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China |
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Abstract We studied the effects of alfalfa saponin extract (ASE) on low density lipoprotein receptor (Ldlr), liver X receptor α (LXRα), and farnesoid X receptor (FXR) in normal and hyperlipidemic Buffalo rat liver (BRL) cells. Normal and hyperlipidemic BRL cells were divided into eight groups: normal, or normal cells treated with 50, 100, and 150 mg/L ASE, hyperlipidemic, or hyperlipidemic cells treated with 50, 100, and 150 mg/L ASE. After treatment for 24 h, Ldlr, LXRα, and FXR mRNA expression levels were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Data showed that mRNA expression of Ldlr in normal BRL cells was significantly up-regulated by ASE treatment and mRNA expressions of LXRα and FXR were significantly down-regulated both in normal and hyperlipidemic BRL cells after ASE treatment. Thus, ASE might ameliorate hepatic steatosis by regulating genes involved in cholesterol metabolism, including up-regulation of Ldlr as well as down-regulation of LXRα and FXR.
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Received: 30 November 2014
Published: 08 June 2015
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Cite this article:
Xin-ping Liang, Dong-qiang Zhang, Yan-yan Chen, Rui Guo, Jie Wang, Cheng-zhang Wang, Ying-hua Shi. Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2015, 16(6): 479-486.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1400343 OR http://www.zjujournals.com/xueshu/zjus-b/Y2015/V16/I6/479
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