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| Inactivation of infectious bursal disease virus by binary ethylenimine and formalin |
| Habib M., Hussain I., Fang W.H., Rajput Z.I., Yang Z.Z., Irshad H. |
| Department of Veterinary Microbiology, University of Agriculture, Faisalabad 38040, Pakistan; Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China; Department of Livestock and Fisheries, Animal Husbandry Sindh, Hyderabad 71000, Pakistan |
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Abstract In this experiment conducted to study the inactivation dynamics of infectious bursal disease virus (IBDV) by binary ethylenimine (BEI) in comparison with formalin, IBDV was isolated from the bursa of infected chickens and its confirmation was done by agar gel precipitation test. Viral suspensions were subjected to inactivation with BEI and formalin for pre-set time intervals. BEI was employed at concentrations of 0.001 and 0.002 mol/L while formalin was used at 0.1% and 0.2%. Sampling was done at 6, 12, 24, 36 and 48 h of incubation and samples were tested for their inactivation status in 9-day-old embryonated eggs and 3-week-old broiler chickens. IBDV was completely inactivated by 0.001 and 0.002 mol/L BEI after 36 h of incubation at 37 °C, whereas formalin at 0.1% and 0.2% concentrations inactivated IBDV in 24 h.
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Received: 29 October 2005
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