|
Chinese public understanding of the use of agricultural biotechnology—A case study from Zhejiang Province of China
Lü Lan
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(4 ): 2-.
https://doi.org/10.1631/jzus.2006.B0257
This study explores the Chinese public’s perceptions of, and attitudes to, agriculture and food applications of biotechnology; and investigates the effect of socio-demographic factors on attitudes. A questionnaire survey and interviews were used in an attempt to combine quantitative analysis with qualitative review. The main finding of this study is that the Chinese population has a superficial, optimistic attitude to agricultural biotechnology; and that, in accordance with public attitudes, a cautious policy, with obligatory labelling, should be adopted. The study reveals that education is the factor among socio-demographic variables with the strongest impact on public attitudes. Higher education leads to a more positive evaluation of GM (genetically modified) foods and applications of biotechnology with respect to usefulness, moral acceptability, and suitability for encouragement. In addition, public attitudinal differences depend significantly on area of residence. Compared with their more urban compatriots, members of the public in less developed areas of China have more optimistic attitudes, perceive more benefits, and are more risk tolerant in relation to GM foods and agricultural biotechnology. Finally we obtained a very high rate of “don’t know” answers to our survey questions. This suggests that many people do not have settled attitudes, and correspondingly, that the overall public attitude to agricultural biotechnology and GM foods in China is at present somewhat unstable.
|
|
Intra-specific genetic relationship analyses of Elaeagnus angustifolia based on RP-HPLC biochemical markers
Wang Qiang, Ruan Xiao, Huang Jun-Hua, Xu Ning-Yi, Yan Qi-Chuan
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(4 ): 4-.
https://doi.org/10.1631/jzus.2006.B0272
Elaeagnus angustifolia Linn. has various ecological, medicinal and economical uses. An approach was established using RP-HPLC (reversed-phase high-performance liquid chromatography) to classify and analyse the intra-specific genetic relationships of seventeen populations of E. angustifolia, collected from the Xinjiang areas of China. Chromatograms of alcohol-soluble proteins produced by seventeen populations of E. angustifolia, were compared. Each chromatogram of alcohol-soluble proteins came from a single seed of one wild plant only. The results showed that when using a Waters Delta Pak. C18, 5 μm particle size reversed phase column (150 mm×3.9 mm), a linear gradient of 25%~60% solvent B with flow rate of 1 ml/min and run time of 67 min, the chromatography yielded optimum separation of E. angustifolia alcohol-soluble proteins. Representative peaks in each population were chosen according to peak area and occurrence in every seed. The converted data on the elution peaks of each population were different and could be used to represent those populations. GSC (genetic similarity coefficients) of 41% to 62% showed a medium degree of genetic diversity among the populations in these eco-areas. Cluster analysis showed that the seventeen populations of E. angustifolia could be divided into six clusters at the GSC=0.535 level and indicated the general and unique biochemical markers of these clusters. We suggest that E. angustifolia distribution in these eco-areas could be classified into six variable species. RP-HPLC was shown to be a rapid, repeatable and reliable method for E. angustifolia classification and identification and for analysis of genetic diversity.
|
|
Impact of elevated CO2 concentration under three soil water levels on growth of Cinnamomum camphora
Zhao Xing-Zheng, Wang Gen-Xuan, Shen Zhu-Xia, Zhang Hao, Qiu Mu-Qing
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(4 ): 6-.
https://doi.org/10.1631/jzus.2006.B0283
Forest plays very important roles in global system with about 35% land area producing about 70% of total land net production. It is important to consider both elevated CO2 concentrations and different soil moisture when the possible effects of elevated CO2 concentration on trees are assessed. In this study, we grew Cinnamomum camphora seedlings under two CO2 concentrations (350 μmol/mol and 500 μmol/mol) and three soil moisture levels [80%, 60% and 40% FWC (field water capacity)] to focus on the effects of exposure of trees to elevated CO2 on underground and aboveground plant growth, and its dependence on soil moisture. The results indicated that high CO2 concentration has no significant effects on shoot height but significantly impacts shoot weight and ratio of shoot weight to height under three soil moisture levels. The response of root growth to CO2 enrichment is just reversed, there are obvious effects on root length growth, but no effects on root weight growth and ratio of root weight to length. The CO2 enrichment decreased 20.42%, 32.78%, 20.59% of weight ratio of root to shoot under 40%, 60% and 80% FWC soil water conditions, respectively. And elevated CO2 concentration significantly increased the water content in aboveground and underground parts. Then we concluded that high CO2 concentration favours more tree aboveground biomass growth than underground biomass growth under favorable soil water conditions. And CO2 enrichment enhanced lateral growth of shoot and vertical growth of root. The responses of plants to elevated CO2 depend on soil water availability, and plants may benefit more from CO2 enrichment with sufficient water supply.
|
|
Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)
Rao Abdul Qayyum, Hussain S. Sarfraz, Shahzad M. Saqib, Bokhari S. Yassir Abbas, Raza M. Hashim, Rakha Allah, Majeed A., Shahid A. Ali, Saleem Zafar, Husnain Tayyab, Riazuddin S.
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(4 ): 7-.
https://doi.org/10.1631/jzus.2006.B0291
Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypocotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods.
|
|
Band structures of TiO2 doped with N, C and B
Xu Tian-Hua, Song Chen-Lu, Liu Yong, Han Gao-Rong
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(4 ): 8-.
https://doi.org/10.1631/jzus.2006.B0299
This study on the band structures and charge densities of nitrogen (N)-, carbon (C)- and boron (B)-doped titanium dioxide (TiO2) by first-principles simulation with the CASTEP code (Segall et al., 2002) showed that the three 2p bands of impurity atom are located above the valence-band maximum and below the Ti 3d bands, and that along with the decreasing of impurity atomic number, the fluctuations become more intensive. We cannot observe obvious band-gap narrowing in our result. Therefore, the cause of absorption in visible light might be the isolated impurity atom 2p states in band-gap rather than the band-gap narrowing.
|
|
Sorption of wastewater containing reactive red X-3B on inorgano-organo pillared bentonite
Zeng Xiu-Qiong
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(4 ): 11-.
https://doi.org/10.1631/jzus.2006.B0314
Bentonite is a kind of natural clay with good exchanging ability. By exchanging its interlamellar cations with various soluble cations, such as quaternary ammonium cations and inorganic metal ions, the properties of natural bentonite can be greatly improved. In this study, hexadecyltrimethylammonium bromide (HDTMA), CaCl2, MgCl2, FeCl3, AlCl3 were used as organic and inorganic pillared materials respectively to produce several kinds of Ca-, Mg-, Fe-, Al-organo pillared bentonites. Sorption of reactive red X-3B on them was studied to determine their potential application as sorbents in wastewater treatment. The results showed that these pillared bentonites had much improved sorption properties, and that the dye solutions’ pH value had some effect on the performance of these inorgano-organo pillared bentonites. Isotherms of reactive X-3B on these pillared bentonites suggested a Langmuir-type sorption mechanism.
|
|
Inactivation of infectious bursal disease virus by binary ethylenimine and formalin
Habib M., Hussain I., Fang W.H., Rajput Z.I., Yang Z.Z., Irshad H.
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(4 ): 12-.
https://doi.org/10.1631/jzus.2006.B0320
In this experiment conducted to study the inactivation dynamics of infectious bursal disease virus (IBDV) by binary ethylenimine (BEI) in comparison with formalin, IBDV was isolated from the bursa of infected chickens and its confirmation was done by agar gel precipitation test. Viral suspensions were subjected to inactivation with BEI and formalin for pre-set time intervals. BEI was employed at concentrations of 0.001 and 0.002 mol/L while formalin was used at 0.1% and 0.2%. Sampling was done at 6, 12, 24, 36 and 48 h of incubation and samples were tested for their inactivation status in 9-day-old embryonated eggs and 3-week-old broiler chickens. IBDV was completely inactivated by 0.001 and 0.002 mol/L BEI after 36 h of incubation at 37 °C, whereas formalin at 0.1% and 0.2% concentrations inactivated IBDV in 24 h.
|
|
Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis
Sun Huai-Chang, Xue Fang-Ming, Qian Ke, Fang Hao-Xia, Qiu Hua-Lei, Zhang Xin-Yu, Yin Zhao-Hua
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(4 ): 13-.
https://doi.org/10.1631/jzus.2006.B0324
To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.
|
13 articles
|