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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2005, Vol. 6 Issue ( 7): 9-    DOI: 10.1631/jzus.2005.B0656
    
Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase
YANG Hua, ZHENG Shu, MEIJER Laurent, LI Shi-min, LECLERC Sophie, YU Lin-lin, CHENG Jin-quan, ZHANG Su-zhan
Cancer Institute, Zhejiang University, Hangzhou 310009, China; Departments of Pathology and Interdisciplinary Oncology, University of South Florida, School of Medicine and H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA; Cell Cycle Laborator CNRS, Station Biologique, BP 74, 29682 Roscoff Cedex, Bretagne, France; School of Pharmaceutical Science, Zhejiang University, Hangzhou 310031, China
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Abstract  Objective: To screen and evaluate the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 phosphatase. Methods: The affinity chromatography purified glutashione-S-transferase/Cdc25A phosphatase fusion protein and Cdc2/cyclin B from the extracts of starfish M phase oocytes are used as the cell cycle-specific targets for screening the antimitotic constituents. We tested 9 extracts isolated from the Chinese medicinal herbs and vegetables including the agents currently used in cancer treatment by measuring the inhibition of Cdc25A phosphatase and Cdc2 kinase activity. The antitumor activity of the extracts was also evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry. Results: Cdc25A inhibitory activity and antitumor activity are detected in the extracts isolated from three Chinese medicinal herbs Agrimona pilosa; Herba solani lyrati; Galla chinesis. Conclusion: We found three extracts isolated from Chinese medicinal herbs have potential inhibitory activity of Cdc25 phosphatase using a highly specific mechanism-based screen assay for antimitotic drug discovery.

Key words： Chinese medicinal herbs      Cdc25 phosphatase inhibition      Cell cycle      Anti-tumor activity     
Received: 25 September 2004     
CLC:  R657.3  
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YANG Hua
ZHENG Shu
MEIJER Laurent
LI Shi-min
LECLERC Sophie
YU Lin-lin
CHENG Jin-quan
ZHANG Su-zhan
Cite this article:

YANG Hua, ZHENG Shu, MEIJER Laurent, LI Shi-min, LECLERC Sophie, YU Lin-lin, CHENG Jin-quan, ZHANG Su-zhan. Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 7): 9-.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2005.B0656     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2005/V6/I 7/9

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