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Waste Disposal & Sustainable Energy
Waste Disposal & Sustainable Energy  2019, Vol. 1 Issue (3): 213-226    DOI: 10.1007/s42768-019-00016-w
    
利用龙舌兰芽孢杆菌G9促进含培养基木质纤维素类废弃物生产纤维素酶的统计优化研究
Mudasir A. Dar1, Kiran D. Pawar2, Jyoti M. Chintalchere1, Radhakrishna S. Pandit1
1 Department of Zoology, Savitribai Phule Pune University, Ganeshkhind, Pune, Maharashtra 411007, India  2 School of Nanoscience and Biotechnology, Shivaji University, Vidyanagar, Kolhapur, Maharashtra 416004, India
Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by Bacillus tequilensis G9
Mudasir A. Dar1, Kiran D. Pawar2, Jyoti M. Chintalchere1, Radhakrishna S. Pandit1
1 Department of Zoology, Savitribai Phule Pune University, Ganeshkhind, Pune, Maharashtra 411007, India  2 School of Nanoscience and Biotechnology, Shivaji University, Vidyanagar, Kolhapur, Maharashtra 416004, India
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摘要： 现代文明对能源的需求不断增加，化石燃料迅速减少，这表明生物燃料是一种可再生的替代品。然而，与生物燃料生产相关的更高成本是其商业化的主要瓶颈。本研究表明，使用统计方法，称为响应面法（RSM），来研究tequilensis G9 纤维素酶最大产量的最佳参数。RSM分析的Plackett-Burman设计（PB）表明，草秆（GS）浓度、pH、FeSO4、接种物、MgSO4、培养期和NH4Cl是影响纤维素酶产量的显著变量。此外，提出了以B. tequilensis G9生产纤维素酶的最佳培养基，最有影响的参数，即GS浓度为底物、FeSO4、pH、接种量等，通过中心组合设计法（CCD）进行微调。CCD分析表明，8%的底物浓度、1.5%的接种量、10ppm的FeSO4和培养基中的pH值为5.5是产酶的最佳条件。场发射扫描电镜分析表明，处理后的GS结构发生了改变，表现为B.tequilensis G9引起的明显解构。部分纯化的纤维素酶蛋白的产率为21%，分子量在30～97kda之间。在我们的研究中，tequilensis G9芽孢杆菌的纤维素酶产量增加，这表明它在许多工业过程中的应用，如生物炼制、生物燃料等。
关键词： tequilensis G9芽孢杆菌;  纤维素酶生产;  Plackett-Burman设计;  中心组合设计;  RSM;  糖化    
Abstract: The ever increasing energy demands of modern civilization and rapidly dwindling fossil fuels point towards a renewable substitute like biofuels. However, higher costs associated with biofuel productions is the major bottleneck for its commercialization. The present study demonstrates the use of a statistical approach called response surface methodology (RSM) to investigate the optimum parameters for maximum production of cellulase by Bacillus tequilensis G9. The Plackett–Burman design (PB) of the RSM analysis indicated grass straw (GS) concentration, pH, FeSO4, inoculum, MgSO4, incubation period and NH4Cl as significant variables that influence the cellulase production. Further, to propose the best medium for the maximum production of cellulase by B. tequilensis G9, the most influential parameters, namely concentrations of GS as substrate, FeSO4, pH, inoculum size, etc. were fine-tuned by central composite design (CCD) involving four factors and five levels. The CCD analysis demonstrated 8% substrate concentration, 1.5% of inoculum along with 10 ppm FeSO4 and a pH of 5.5 in media as optimum conditions for highest enzyme production. The field emission scanning electron microscopic analysis of the treated GS showed structural alterations depicting significant deconstruction caused by B. tequilensis G9. The yield of the partially purified cellulase proteins were found to be 21% revealing molecular mass between 30 and 97 kDa. The enhanced cellulase production by B. tequilensis G9 demonstrated in our study brands its applications in many industrial processes like biorefinery, biofuels, etc.
Key words: Bacillus tequilensis G9    Cellulase production    Plackett–Burman design    Central composite design    RSM    Saccharifcation
收稿日期: 2019-05-18 出版日期: 2020-01-14
通讯作者: Kiran D. Pawar     E-mail: pawarkiran1912@gmail.com
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Mudasir A. Dar
Kiran D. Pawar
Jyoti M. Chintalchere
Radhakrishna S. Pandit

引用本文:

Mudasir A. Dar, Kiran D. Pawar, Jyoti M. Chintalchere, Radhakrishna S. Pandit. Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by Bacillus tequilensis G9. Waste Disposal & Sustainable Energy, 2019, 1(3): 213-226.

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http://www.zjujournals.com/wdse/CN/10.1007/s42768-019-00016-w    或    http://www.zjujournals.com/wdse/CN/Y2019/V1/I3/213

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