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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)
动物科学与动物医学     
艾叶对热应激蛋鸡抗氧化功能、产蛋性能和蛋品质的影响
徐红蕊, 陈小连, 时建青, 宋琼莉
1.河南牧业经济学院,郑州450008; 2.江西省农业科学院畜牧兽医研究所,南昌330200; 3.新希望六和股份有限公司,北京100102
Effects of Artemisia argyi on the antioxidant activity, laying performance and egg quality in laying hens under heat stress
XU Hongrui, CHEN Xiaolian2*, SHI Jianqing3, SONG Qiongli2
(1. Henan University of Animal Husbandry and Economy, Zhengzhou 450008, China; 2. Institute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China; 3. New Hope Liuhe Co., Ltd, Beijing 100102, China)
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摘要： 为了评价艾叶缓解蛋鸡热应激效应及适宜添加水平,本研究测定了艾叶提取物中抗氧化成分含量及体外抗氧化性能,研究了艾叶对热应激条件下蛋鸡抗氧化功能、产蛋性能和蛋品质的影响。试验选用720只38周龄海兰褐蛋鸡,随机分为6组,每组6个重复,每重复20只,即常温对照组、高温对照组、常温艾叶组、高温低艾叶组、高温中艾叶组、高温高艾叶组。常温和高温对照组饲喂基础饲粮,常温艾叶组在基础饲粮中添加3.0%艾叶粉,高温艾叶组分别在基础饲粮中添加1.0%、2.0%、3.0%艾叶粉。预试期为7 d,正试期21 d,记录生产性能指标并测定鸡蛋品质,每7 d测定1次血清抗氧化及应激指标。结果显示,艾叶具有较高的抗氧化成分和清除自由基能力,总黄酮含量为87.78 mg/g,总多酚含量为126.57 mg/g,维生素C含量为3.12 mg/g,清除羟基自由基、超氧阴离子自由基及DPPH自由基的半数有效剂量分别为356.86 μg/mL、76.84 μg/mL、293.67 μg/mL。在常温条件下,蛋鸡日粮中添加艾叶对蛋鸡生产性能、产蛋性能无显著影响(P>0.05);在高温应激条件下,蛋鸡采食量和产蛋率显著降低,料蛋比升高(P<0.05),在蛋鸡饲料中添加不同水平的艾叶14 d时,与高温对照组相比,蛋鸡采食量无显著变化(P>0.05),血清超氧化物歧化酶和谷胱甘肽过氧化物酶活力显著提高,丙二醛及皮质醇含量显著降低(P<0.05);产蛋率提高及料蛋比降低(P<0.05),且能显著改善蛋黄颜色、降低胆固醇含量(P<0.05)。试验结果表明,艾叶具有较好的体内外抗氧化能力,在热应激条件下蛋鸡日粮中添加2%的艾叶可以一定程度增强蛋鸡抗热应激能力,提高蛋鸡产蛋性能并改善鸡蛋品质。
Abstract: Poultry production has been puzzled by the problem of heat stress in laying hens for a long time. Some Chinese herbal medicine had been reported to improve the ability of anti-heat stress in hens. Artemisia argyi is a kind of abundant resource of herbal medicine in China and was approved clinically to be safe and reliable in human and animals with the property of anti-inflammation and antioxidation. Few studies have reported the effects of A. argyi on laying hens under heat stress. In order to alleviate the heat-stressed effect and maintain the normal laying performance in laying hens under heat stress by nutrition this study explored the effects of A. argyi on antioxidant activity, laying performance and egg quality in laying hens under heat stress.
　　Seven hundred and twenty Hy-Line Brown layers (38-week-old) were divided into 6 groups with 6 replicates per group and 20 hens per replicate according to the temperature and A. argyi level, including normal temperature (control group) and normal temperature with A. argyi group, high temperature stress group and three A. argyi groups in high temperature. The two control groups were fed with a basal diet. Normal temperature with A. argyi group was fed with basal diet with 3% of A. argyi. High temperature treatments were fed with 1%, 2% and 3% of A. argyi, respectively. The preliminary trial period lasted 7 days and trial period lasted 21 days. The antioxidant ingredient and scavenging rates of A. argyi extracts for hydroxide radical, superoxide anion radical and DPPH radical were determined. The laying performance and egg quality were recorded, and the antioxidant indexes and cortisol in serum were determined every 7 days.
　　The results showed that A. argyi has higher antioxidant ingredient and better antioxidant capacity. The contents of flavonoids, total polyphenols, and vitamin C were 87.78 mg/g,126.57 mg/g, and 3.12 mg/g, respectively. The median effective dose of A. argyi extracts for scavenging hydroxide radical, superoxide anion radical and DPPH radical was 356.86 μg/mL， 76.84 μg/mL， 293.67 μg/mL, respectively. There was no significant effect of supplementation of A. argyi on the growth and laying performance in hens under normal condition (P＞0.05). Under high temperature, the hens suffered heat stress showed lower daily feed intake, lower laying rate and higher feed to egg ratio. Supplementation of A. argyi in laying hens under heat stress didn’t affect the daily feed intake compared with high temperature stress group (P>0.05), but significantly improved the antioxidant enzyme activity in serum, reduced the content of malondialdehyde and cortisol (P<0.05), increased laying rate, reduced feed to egg ratio (P<0.05), and significantly improved the yolk color and cholesterol levels (P<0.05).
　　The results indicate that A. argyi has good antioxidant effect in vitro and in vivo and can increase the antioxidant activity and improve egg quality through relieving heat stress. According to the effect and cost, the proper supplementation level of A. argyi in laying hens under heat stress is 2%.
出版日期: 2017-01-25
CLC:  S 831.5　  
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宋琼莉
时建青
徐红蕊
陈小连

引用本文:

徐红蕊, 陈小连, 时建青, 宋琼莉. 艾叶对热应激蛋鸡抗氧化功能、产蛋性能和蛋品质的影响[J]. 浙江大学学报(农业与生命科学版), 10.3785/j.issn.1008-9209.2016.03.172.

XU Hongrui, CHEN Xiaolian, SHI Jianqing, SONG Qiongli. Effects of Artemisia argyi on the antioxidant activity, laying performance and egg quality in laying hens under heat stress. Journal of Zhejiang University (Agriculture and Life Sciences), 10.3785/j.issn.1008-9209.2016.03.172.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2016.03.172    或    http://www.zjujournals.com/agr/CN/Y2017/V43/I1/113

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