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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)
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朝鲜孢囊线虫--浙江省孢囊线虫新记录种
朱红雪, 田忠玲, 蔡瑞杭, 李晓琳, 郑经武
浙江大学农业与生物技术学院生物技术研究所,杭州310058
Heterodera koreana, a new record of cyst-forming nematodes in Zhejiang Province, China
ZHU Hongxue, TIAN Zhongling, CAI Ruihang, LI Xiaolin, ZHENG Jingwu*
(Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China)
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摘要： 在2015—2016年间对浙江省孢囊线虫的种类及分布进行了调查,从浙江省临安市雷竹根围采集到一个孢囊线虫群体,采用形态学和分子生物学方法对其进行了鉴定。利用光学显微镜和扫描电子显微镜对孢囊和2龄幼虫(J2)的形态进行系统的观察和测量,主要形态特征为：孢囊近圆形,有明显的短颈和阴门锥,无下桥、泡囊突和膜孔,阴门裂长45.3 μm;2龄幼虫体长528.5 μm,头部缢缩不明显,具2个唇环数,口针长19.6 μm;侧区具3条侧线;尾圆锥形,末端尖细,尾长75.4 μm,透明尾占尾长的55%～75%。对该线虫群体rDNA的内转录间隔区(internal transcribed spacer,ITS)和28S D2D3区进行特异性扩增,分别得到1 006 bp和786 bp的片段,与朝鲜孢囊线虫(Heterodera koreana)的序列相似度分别高达99.7％和99.6%;综合形态学、分子鉴定及系统进化分析等,将分离自雷竹的孢囊线虫群体鉴定为朝鲜孢囊线虫,系浙江省孢囊线虫的新记录种。
Abstract: Cyst-forming nematode is an important plant parasitic nematode group, in which over 80 species were reported and described. There are many economically important cyst-forming nematodes species in China, such as Heterodera glycines, Heterodera avenae, and Heterodera filipjevii. During 2015—2016, a survey for cyst-forming nematodes in Zhejiang, eastern China, revealed the distribution of a cyst-forming nematode population from Phyllostachys praecox in Lin'an, Zhejiang, eastern China. Morphological characteristics and morphometrics of cysts and second-stage juveniles (J2s) were observed and measured using light microscope and scanning electron microscope. Cysts were characterized by appearing light to dark brown in coloration, globose to subspherical shape of body contour, distinct neck and cone, lacking underbridge, bullae and fenestrae, body length ranging from 575.0 to 1 230.0 μm, body width ranging from 397.0 to 872.0 μm, and vulva slit length ranging from 36.7 to 50.9 μm.
　　J2 was characterized by hemispherical labial region, nearly continuous with round body contour and stylet knobs, three incisures in lateral field, tail tapering and tail hyaline region accounting for 55%—75%, body length ranging from 426.3 to 608.0 μm; body width ranging from 15.9 to 29.3 μm; stylet length ranging from 17.7 to 21.9 μm; dorsal gland orifice ranging from 4.3 to 6.2 μm; tail length ranging from 68.7 to 84.5 μm. Amplification of the ITS and D2/D3 fragments of the cyst-forming nematodes from bamboo with universal primers AB28 (ATATGCTTAAGTTCAGCGGGT) and TW81 (GTTTCCGTAGGTGAACCTGC), D2A (ACAAGTACCGTGA GGGAAAGTTG) and D3B (TCGGAAGGAACCAGCTACTA) yielded PCR fragments with sizes of 1 006 bp (GenBank: KX640828) and 786 bp (GenBank: KX640827), respectively, the sequences of ITS and D2/D3 of cyst nematodes parasitizing on bamboo were 99.7% and 99.6% similar to that of Heterodera koreana deposited in GenBank, respectively. Combined with morphological, morphometrics and molecular phylogeny analysis, the results showed that the cyst nematodes parasitizing on bamboo from Lin'an, Zhejiang, China is H. koreana, is a new record in Zhejiang, Eastern China.
出版日期: 2017-01-25
CLC:  Q 959.17  
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蔡瑞杭
李晓琳
郑经武
朱红雪
田忠玲

引用本文:

朱红雪, 田忠玲, 蔡瑞杭, 李晓琳, 郑经武. 朝鲜孢囊线虫--浙江省孢囊线虫新记录种[J]. 浙江大学学报(农业与生命科学版), 10.3785/j.issn.1008-9209.2016.10.071.

ZHU Hongxue, TIAN Zhongling, CAI Ruihang, LI Xiaolin, ZHENG Jingwu. Heterodera koreana, a new record of cyst-forming nematodes in Zhejiang Province, China. Journal of Zhejiang University (Agriculture and Life Sciences), 10.3785/j.issn.1008-9209.2016.10.071.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2016.10.071    或    http://www.zjujournals.com/agr/CN/Y2017/V43/I1/81

[1] . 山西省小麦孢囊线虫的形态学和分子特征分析[J]. 浙江大学学报(农业与生命科学版), 2012, 38(5): 566-574.
[2] . 南京毛刺线虫（Trichodorus nanjingensis）浙江群体与北京群体的形态学及分子特征比较[J]. 浙江大学学报(农业与生命科学版), 2012, 38(5): 542-550.