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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2013, Vol. 39 Issue (1): 56-    DOI: 10.3785/j.issn.1008-9209.2012.02.213
农业科学     
涝胁迫对不同丹参品系苗期保护酶活性及脂质过氧化作用的影响
路萍1, 张 利1*, 王 萌2, 王涛1, 姜媛媛1
(1.四川农业大学 生命科学与理学院,四川 雅安 625014;2.四川农业大学 农学院,成都 611130）
Effects of water stress on protective enzyme activities and lipid peroxidation in different Salvia miltiorrhiza Bunge varieties at seedling stage
LU Ping1, ZHANG Li1*, WANG Meng2, WANG Tao1,  JIANG Yuanyuan1
(1. College of Biology and Science, Sichuan Agricultural University, Ya′an, Sichuan 625014, China; 2. College of Agriculture, Sichuan Agricultural University, Chengdu 611130, China
)
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摘要： 以四川省中江县6个丹参品系幼苗为试材，采用土培模拟涝害的方法，研究涝胁迫对丹参幼苗叶片超氧化物歧化酶（superoxide dismutase,SOD）、过氧化物酶（peroxidase,POD）、过氧化氢酶（catalase,CAT）活性及游离脯氨酸（proline,Pro）质量分数和丙二醛（malondialdehyde,MDA）质量摩尔浓度的变化。结果表明：涝胁迫5 d作为模拟涝胁迫的最佳条件。在受到涝胁迫时，组培大叶丹参的SOD和CAT活性最高，组培小叶丹参的最低；矮秆大叶丹参的POD活性最高，组培小叶丹参的最低；四倍体小叶丹参的Pro质量分数和MDA的质量摩尔浓度最高，中江野生丹参的Pro质量分数最低；组培大叶丹参、高秆大叶丹参和矮秆大叶丹参的MDA质量摩尔浓度相同，且低于其他3个品系。对各项生理生化指标的相关性分析表明，各指标相关性不显著，彼此呈独立作用态势，均可作为鉴定丹参抗涝性的生理指标。
Abstract: The dried root and rhizome of Salvia miltiorrhiza Bunge (Lamiaceae, Salvia), one of the popular traditional Chinese medicine (TCM), is officially listed in Chinese pharmacopoeia under the name of Danshen. S. miltiorrhiza have been widely used for treatment of coronary heart disease, cerebrovascular disease, neurasthenic insomnia, bone loss, liver fibrosis, hepatocirrhosis and chronic renal failure. Waterlogging is abiotic stress that influences species composition and productivity in numerous plant communities worldwide. For most crops, excess water is a major constraint to productivity in many regions and situations, and the inability of crops to withstand low oxygen conditions in the root zone will lead to yield losses. Zhongjiang County was the genuine producing areas with a long history
 for the plant of Danshen. But now, the  effect mechanism of water stress on S. miltiorrhiza was little  known. The objective of this study was to elucidate the physiological responses of different S. miltiorrhiza under water stress. The finite element method can be used as academic base for flooding resistance mechanisms, and provide evidence for breeding S. miltiorrhiza varieties and
improving the  cultivation technology of resistance to waterlogging. The experiment was conducted in  2010—2011 at the experimental station of Sichuan Agricultural University, Ya′an, Sichuan Province, China. Bigleaf dwarf S. miltiorrhiza, bigleaf tissue cultured S. miltiorrhiza, tetraploid lobular S. miltiorrhiza, tall bigleaf S. miltiorrhiza, Zhongjiang wild S. miltiorrhiza, lobular tissue cultured  S. miltiorrhiza were obtained from Zhongjiang County, Sichuan Province. All materials were grown in pots containing a 2:1 mixture of garden soil and vermiculite without any added fertilizer, and were maintained in a rain shelter. The waterlogging treatment was mimicked by treating pot plants with flooding. Investigations were carried out on the changes of malondialdehyde (MDA) content, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) activities and proline (Pro) content of six S. miltiorrhiza varieties. The result indicated that waterlogging stress of 5 days was the optimum condition for the simulation of waterlogging stress. Each variety had different physiological and biochemical response to waterlogging stress. Under the flooding stress, SOD and CAT activity of tissue cultured bigleaf S. miltiorrhiza was the highest, and that of tissue cultured  lobular S. miltiorrhiza was the lowest; POD activity of dwarf bigleaf S. miltiorrhiza was the highest, and that of tissue cultured  lobular S. miltiorrhiza was the lowest; Tetraploid lobular S. miltiorrhiza contained the most contents of Pro and MDA, while Zhongjiang wild S. miltiorrhiza contained the least content of Pro; Tissue cultured  bigleaf S. miltiorrhiza, tall bigleaf S. miltiorrhiza and Dwarf bigleaf S. miltiorrhiza contained the same content of MDA which was lower than the other three varieties. Correlation analysis showed that there was no prominent correlation among all these indexes. Actually, they have independent functions. Therefore, each one can be regarded as physiological index of water tolerance for S. miltiorrhiza. In conclusion, the research results suggested that: i) Waterlogging stress of 5 days was the optimum condition for the simulation of waterlogging stress. ii) Each variety of S. miltiorrhiza  had different physiological and biochemical response to waterlogging stress. iii) Each index can be regarded as physiological trait of water tolerance for S. miltiorrhiza seedlings.
出版日期: 2013-01-20
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引用本文:

路萍1, 张 利1*, 王 萌2, 王涛1, 姜媛媛1. 涝胁迫对不同丹参品系苗期保护酶活性及脂质过氧化作用的影响[J]. 浙江大学学报(农业与生命科学版), 2013, 39(1): 56-.

LU Ping1, ZHANG Li1*, WANG Meng2, WANG Tao1, JIANG Yuanyuan1. Effects of water stress on protective enzyme activities and lipid peroxidation in different Salvia miltiorrhiza Bunge varieties at seedling stage. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(1): 56-.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2012.02.213    或    http://www.zjujournals.com/agr/CN/Y2013/V39/I1/56

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