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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2012, Vol. 38 Issue (5): 542-550    DOI: 10.3785/j.issn.1008-9209.2011.10.051
生物科学与技术     
南京毛刺线虫（Trichodorus nanjingensis）浙江群体与北京群体的形态学及分子特征比较
李戌清1,2， 林晓佳3， 郑经武1*
1.浙江大学 农业与生物技术学院 生物技术研究所，浙江 杭州 310058；2.杭州市农业科学研究院，浙江 杭州 310024；
3.浙江出入境检验检疫局，浙江 杭州310016
Morphological and molecular characterization of Trichodorus nanjingensis populations occurring in Zhejiang and Beijing, China  
LI Xuqing1,2, LIN Xiaojia3, ZHENG Jingwu1*
1. Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; 2. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China; 3. Zhejiang EntryExit Inspection and Quarantine Bureau, Hangzhou 310016, China
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摘要： 对采集自国内13省（市）的1 546份土壤样品进行线虫分离和鉴定，从北京和浙江2省(市)的果园、桑园、观赏性花木等多年生植物的根围土样中分离到23个南京毛刺线虫群体，其中北京群体检出率占北京样品采集总量的39.1%，表明南京毛刺线虫是北京市多年生植物根围寄生线虫优势种；通过对浙江群体与北京群体的形态及测量值比较，发现不同地理群体间的形态测量值差异很小，但北京美人梅（代码129）群体的2条雄虫和水栒子（代码088）群体的1条雄虫仅具1枚腹中颈乳突；各群体18S RNA区扩增序列与GenBank登录序列AJ439579和AJ439580的相似百分比达到99.2%～100.0%；对上述南京毛刺线虫群体rDNA ITS1区、ITS2区和28S RNA基因中D2D3区序列比较和酶切分析显示，该3段序列在南京毛刺线虫种内群体间都较为保守。
Abstract: Stubby root nematodes (Trichodorus spp.) are a group of ectoparasitic nematodes that not only affect root growth but also lead to root stagnation of an extensive range of crops by their feeding on plant root cells, and the importance  is that some of which are virus vectors and can transmit viruses to many economically important crops such as flowers, vegetables, crops, fruit trees, etc. Until now, more than 50 species have been described in this genus all over the world, by using traditional diagnosis of nematode species based on examination of morphology and  morphometrics, which need special taxonomists, is timeconsuming and laborious. Recent years, comparative analysis of rDNA genes and their associated spacer regions is becoming a popular tool for species and subspecies identification and construction of phylogenetic trees of many nematode groups, including stubby root nematodes.
Among 1546 soil samples collected from 13 provinces of China, including Beijing, Shandong, Shanxi, Henan, Jiangsu, Anhui, Hunan, Chongqing, Zhejiang, Fujian, Yunnan, Hainan, and Guangdong, 23 populations of Trichodorus nanjingensis were identified from the rhizosphere of Chinese mulberry, ornamental flowers in Zhejiang Province, and orchards in Beijing, by systematical study based on the morphological and rDNA characters.
The results showed that the detection rate of T. nanjingensis populations from Beijing accounted for 39.1% of the total samples collected in Beijing, which indicated T. nanjingensis as the dominant nematode species in perennial plants of Beijing. Through the morphological and morphometrical comparisons, slight variation between intraspecies was revealed by the populations of T. nanjingensis from Beijing and Zhejiang Province, but two males of population 129 isolated from Prunus blireana and one male of population 088 isolated from Cotoneaster multiflorus from Beijing had one cervical papillae instead of two. The sequence similarity of 18S rDNA between T. nanjingensis populations from Beijing and Zhejiang Province and those submitted in GenBank (AJ439579 and AJ439580) ranged in 99.2%-100.0%. The sequence alignments of ITS1, ITS2 region of rDNA and D2D3 region of 28S RNA gene revealed the consistency between these populations. These results indicate that rDNA sequences can provide useful information for identifying T. nanjingensis from the others.    
出版日期: 2012-09-20
CLC:  Q 959.17  
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[1] . 山西省小麦孢囊线虫的形态学和分子特征分析[J]. 浙江大学学报(农业与生命科学版), 2012, 38(5): 566-574.