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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2012, Vol. 38 Issue (4): 393-399    DOI: 10.3785/j.issn.1008-9209.2012.04.006
生物科学与技术     
应用3种遗传分析方法分析养殖鲤与天然群体的遗传差异
许丽花1， 李巍2， 吴春琳1， 董在杰3， 王成辉1
1.上海海洋大学　农业部水产种质资源与利用重点开放实验室，上海 201306；2.全国水产技术推广站，北京 100125；
3.中国水产科学研究院 淡水渔业研究中心/农业部淡水鱼类遗传育种和养殖生物学重点开放实验室，江苏 无锡　214081
Genetic differentiation of domesticated and wild common carps detected by three genetic analysis methods
XU Lihua1, LI Wei2, WU chunlin1, DONG Zaijie3, WANG Chenghui1
1.Key Laboratory of Aquatic Genetic Resources and Utilization Certificated by Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China; 2. National Fisheries Technical Extension Center, Beijing, 100125, China; 3. Key Open Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture/ Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu 214081, China
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摘要： 应用基于分子标记的主成分分析法、贝叶斯遗传聚类法和遗传重排技术对鲤4个人工选育群体（兴国红鲤、荷包红鲤、玻璃红鲤和建鲤）和2个长江干流天然群体（湖北监利和江苏扬州）的10个微卫星标记结果进行分析，以有效检测人工选育群体间以及选育群体与天然群体间的遗传差异。主成分分析显示，人工选育群体与天然群体存在一定程度的遗传分化，荷包红鲤与天然群体的遗传差异最大，其主成分1（PC1）和主成分2（PC2）解释了总遗传变异的4823％；在贝叶斯遗传聚类分析中，6个群体的最佳聚类数值为4，即兴国红鲤与2个天然群体聚为一类，玻璃红鲤、荷包红鲤和建鲤3个群体分别单独聚为一类；贝叶斯遗传重排分析显示，6个群体的遗传自排率较高，为81％~100％，玻璃红鲤和荷包红鲤的遗传自排率最高，均为100％。研究结果综合表明：4个人工选育群体与天然群体间存在较明显的遗传差异，而且天然群体已受到人工选育群体的遗传影响；这3种方法能很好地检测鲤人工选育群体间、以及选育群体与天然群体间的遗传差异。
Abstract: Based on ten microsatellite loci, the three methods of principal coordinate analysis, Bayesian cluster analysis and genetic assignment techniques were used to detect genetic differentiation of the four domesticated populations (Xingguo red common carp, glass red common carp, purse red common carp and jian common carp) and two wild populations (distributed in two different sections of Yangtze River: Jianli in Hubei and Yangzhou in Jiangsu) of common carp (Cyprinus carpio L.). The principal coordinate  analysis demonstrated that there was obvious genetic differentiation between domesticated and wild common carps, especially between purse red common carp and wild common carp that accounted for 4823％ of total variation according to the first and second principal components. The Bayesian cluster analysis showed four major genetic clusters among six populations, in which xingguo red common carp and the two wild common carp populations consisted of one cluster, and glass red common carp, purse red common carp and jian common carp consisted of one cluster, respectively. The Bayesian genetic assignment technique displayed high selfassignment accuracy for each population (81％100％), especially for glass red common carp and purse red common carp (100％ for each). The present study indicated that there were obvious genetic differentiations  between the domesticated and wild common carps, and wild populations had been influenced by the domesticated strains. As a result, the three methods can effectively detect genetic differentiation not only among domesticated populations, but also between domesticated and wild common carps.
出版日期: 2012-07-25
CLC:  Q346+.5  
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许丽花1
李巍2
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董在杰3
王成辉1

引用本文:

许丽花1， 李巍2， 吴春琳1， 董在杰3， 王成辉1. 应用3种遗传分析方法分析养殖鲤与天然群体的遗传差异[J]. 浙江大学学报(农业与生命科学版), 2012, 38(4): 393-399.

XU Lihua1, LI Wei2, WU chunlin1, DONG Zaijie3, WANG Chenghui1. Genetic differentiation of domesticated and wild common carps detected by three genetic analysis methods. , 2012, 38(4): 393-399.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2012.04.006    或    http://www.zjujournals.com/agr/CN/Y2012/V38/I4/393

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