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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (5): 565-572    DOI: 10.3785/j.issn.1008-9209.2011.05.015
食品科学     
PCR‐TRFLP 研究托盘包装冷却猪肉冷藏过程中的菌相变化
江芸１ ,２, 高峰１, 徐幸莲１, 叶可萍１, 周光宏１
１.南京农业大学国家肉品质量安全控制工程技术研究中心,江苏南京２１００９５;２.南京师范大学金陵女子学院,江苏南京２１００９７
Changes in bacterial communities of tray‐packaged pork during chilled storage analyzed by PCR‐TRFLP .
JIANG Yun１ ,２, GAO Feng１, XU Xing‐lian１, YE Ke‐ping１, ZHOU Guang‐hong１
1 . National Center of Meat Quality and Safety Control , N anjing Agricultural University , Nanjing 210095 , China ;2 . Ginling College , Nanjing Normal University , Nanjing 210097 , China
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摘要： 应用PCR‐末端限制性片段长度多态性分析( PCR‐TRFLP) ,结合１６S rRNA 基因克隆文库和序列分析,研究托盘包装冷却猪肉在４ ℃ 贮藏过程中的菌相变化.结果表明:在整个贮藏过程的肉样TRFLP 图谱中共产生３５种谱带,表明冷却肉中微生物组成十分复杂;对应于假单胞菌( Pseudomonas)的谱带峰在贮藏过程中变化明显,其相对峰面积由０d 的５畅７％至贮藏末期达到８３.４％ ,表明贮藏末期主要优势腐败菌为假单胞菌.
Abstract: PCR‐terminal restriction fragment length polymorphisms ( PCR‐TRFLP) combined with the method of １６S rRNA gene clone library building and sequencing were used to investigate the changes in the bacterial communities of tray‐packaged pork during ４℃ storage . The results showed that ３５terminal restriction fragments ( TRF ) were observed in the TRFLP profiles , indicating that the composition of bacteria population in chilled pork was complex . The peak of the TRF corresponding to Pseudomonas varied significantly during the whole storage , and the relative peak area continued to increase from ５.７％ at day ０to ８３.４％ at the end of monitoring , which indicated that Pseudomonasbecame the predominant bacterial species in the end .
出版日期: 2011-09-20
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江芸
高峰
徐幸莲
叶可萍
周光宏

引用本文:

江芸, 高峰, 徐幸莲, 叶可萍, 周光宏. PCR‐TRFLP 研究托盘包装冷却猪肉冷藏过程中的菌相变化[J]. 浙江大学学报(农业与生命科学版), 2011, 37(5): 565-572.

JIANG Yun, GAO Feng, XU Xing‐lian, YE Ke‐ping, ZHOU Guang‐hong. Changes in bacterial communities of tray‐packaged pork during chilled storage analyzed by PCR‐TRFLP .. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(5): 565-572.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.05.015    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I5/565

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