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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (5): 557-564    DOI: 10.3785/j.issn.1008-9209.2011.05.014
农业科学     
草鱼食用鱼不同颜色肝脏脂肪肝程度的判断
田娟, 文华, 曾令兵, 吴凡, 蒋明, 刘伟
中国水产科学研究院长江水产研究所农业部淡水生物多样性保护与利用重点开放实验室,湖北武汉４３０２２３
Judgment on the degree of fatty liver in different color livers for edible grass carp , Ctenopharyngodon idella .
TIAN Juan , WEN Hua , ZENG Ling‐bing , WU Fan , JIANG Ming , LIU Wei
Key Laboratory of Freshwater Biodiversity Conservation and Utilization , Ministry of Agriculture , Yangtze River Fisheries Research Institute , Chinese Academy of Fishery Sciences , Wuhan 430223 , China
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摘要： 通过测定草鱼( Ctenopharyngodon idella)食用鱼不同颜色肝脏的水分和粗脂肪含量,并运用冰冻组织切片油红O 染色技术对肝细胞进行染色,观察肝细胞脂肪滴的变化,利用Image‐pro plus ６.０ 软件测定其阳性面积率,判断不同颜色肝脏的草鱼食用鱼脂肪肝程度.结果表明:与草鱼正常肝相比,花肝、发白肝和发黄肝的水分含量分别降低６.３０％ 、１０.０１％和１４.１７％ ( P ＜０.０５) ,粗脂肪含量分别升高１９８.２５％ 、３３１.００％ 和４８５.５９％ ( P＜０.０５) ;参照人肝细胞脂肪变性级别的判断标准,正常肝、花肝、发白肝和发黄肝的级别分别评为１.１０ ± ０.３０ 、２.３０ ± ０.２０ 、３.４０ ± ０.５０ 和４.００ ± ０.２０ ;经Image‐pro plus ６.０ 软件分析,花肝、发白肝和发黄肝阳性面积率分别是正常肝的３.９２ 、６.３１ 和７.３２ 倍;以上指标经回归分析得到的回归方程为:肝脏粗脂肪含量(Y ) 与水分( X ) :Y ＝ － １.０８９ ９ X ＋ ８２.０３５ ７ ,R２ ＝ ０.９８９( P＜ ０.０１) ;阳性面积率(Y )与粗脂肪含量( X) :Y ＝ ０.９７０ ２ X － ０.２０９ ０ ,R２ ＝ ０.９７３ ( P ＜ ０.０５ ) ;粗脂肪含量(Y )与肝细胞脂肪变性级别( X) :Y ＝ ３.６６２ ４ X － １.７８８ ６ ,R２ ＝ ０.９８５ ( P ＜ ０.０１ ) ;阳性面积率(Y )与肝细胞脂肪变性级别( X) :Y ＝ ３.６２２ ４ X － ２.１３０ ６ ,R２ ＝ ０.９９６( P＜ ０.０１) .结果证实,冰冻组织切片油红O 染色技术对鱼类脂肪肝的判断具有较好的参考价值,结合阳性面积率与肝脏粗脂肪含量、肝细胞脂肪变性级别具有显著的正相关,本试验建议将草鱼肝细胞阳性面积率＞ ２％ 定义为脂肪肝,其级别评定标准:２％ ～ ７％ 为轻度脂肪肝,７％ ～ １２％ 为中度脂肪肝,＞ １２％ 为重度脂肪肝.
Abstract: The degree of fatty liver in different color livers for edible grass carp ( Ctenopharyngodon idella) was discussed.Liver moisture was determined by freeze‐drying method , and liver crude fat was measured gravimetrically after ether extract using Soxhlet method.The hepatocyte lipid droplets were observed by light microscopy in oil red Ostaining for frozen sections.Based on the criterion of human'fatty liver , the degree of fatty liver in edible grass carp was then determined by the positive area ratio.
Compared with normal livers of grass carp , the moisture contents of motley livers , blushing livers and yellowing livers were decreased by ６.３０％ ,１０.０１％ and １４.１７％ ( P＜ ０.０５) , respectively ; the crude fat contents were increased by １９８.２５％ , ３３１.００％ and ４８５.５９％ ( P＜ ０.０５) , respectively ; the number of
hepatocyte lipid droplets were significantly increased with the peak in yellowing livers , and the positive area ratios were ３.９２ ,６.３１ and ７.３２ times of the normal liver ( P＜ ０.０５) , respectively.The degree of hepatocyte fatty degeneration in normal liver ,motley liver ,blushing liver and yellowing liver was １.１０ ±０.３０ , ２.３０ ± ０.２０ , ３.４０ ± ０.５０ and ４.００ ± ０.２０ , respectively.After doing linear regression analysis ,liver fat contents ( Y ) were negatively correlated with its moisture contents ( X ) : Y ＝ － １.０８９ ９ X ＋８２.０３５ ７ ,R２ ＝ ０.９８９ ( P＜ ０.０１) ; the positive area ratio ( Y ) were positively correlated with its crude fat content ( X) : Y ＝ ０.９７０ ２ X － ０.２０９ ０ ,R２ ＝ ０.９７３ ( P＜ ０.０５) ; the crude fat content (Y ) were positively correlated with the degree of hepatocyte fatty degeneration ( X ) : Y ＝ ３.６６２ ４ X － １.７８８ ６ ,R２ ＝ ０.９８５( P＜ ０.０１ ) ; the positive area ratios ( Y ) were also positively correlated with the degree of hepatocyte fatty degeneration ( X) : Y ＝ ３.６２２ ４ X － ２.１３０ ６ ,R２ ＝ ０.９９６ ( P＜ ０.０１).These results confirm that the oil red O staining method for frozen sections is suitable for evaluating the degree of fish fatty liver.As aresult , according to the positive correlation between the positive area ratio and the crude fat content or the degree of hepatocyte fatty degeneration,it is suggested that the liver in grass carp can be defined as fatty liver when its positive area ratio exceeds ２％.Correspondingly , the degree of fatty liver can be defined as mild when its positive area ratio ranges from ２％t o７％,as medium when ranging from ７％to １２％, and as serious when exceeding １２％ .
出版日期: 2011-09-20
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曾令兵
吴凡
蒋明
田娟
文华
刘伟

引用本文:

田娟, 文华, 曾令兵, 吴凡, 蒋明, 刘伟. 草鱼食用鱼不同颜色肝脏脂肪肝程度的判断[J]. 浙江大学学报(农业与生命科学版), 2011, 37(5): 557-564.

TIAN Juan,WEN Hua,ZENG Ling‐bing,WU Fan,JIANG Ming,LIU Wei. Judgment on the degree of fatty liver in different color livers for edible grass carp , Ctenopharyngodon idella .. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(5): 557-564.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.05.014    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I5/557

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