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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (4): 446-452    DOI: 10.3785/j.issn.1008-9209.2011.04.014
农业科学     
镁缺乏对“不知火”和“椪柑”生理指标、镁分布及叶片超微结构的影响
申 燕1,肖家欣1,2,杨 慧1,张绍铃2
１.安徽师范大学生命科学学院生物环境与生态安全安徽省高校省级重点实验室,安徽 芜湖 ２４１０００ ;２.南京农业大学园艺学院,江苏 南京 ２１００９５
Effects of magnesium deficiency on the physiological indices, magnesium distribution and leaf ultrastructure ofCitrus cultivars  “shiranuhi” and “ponkan”
SHEN Yan1,XIAO Jia-xin1,2,YANG Hui1,ZHANG Shao-ling2
1 . Anhui Province Key Laboratory of Biotic Environment and Ecological Safety , College of Life Sciences , Anhui Normal University , W uhu , A nhui 241000 , China ; 2 .College of Horticulture , Nanjing Agricultural University , Nanjing 210095 , China
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摘要： 采用砂基培养法,研究缺镁胁迫下 不知火"[ Citrus reticulata × ( C . reticulata × C . sinenesis)]和椪柑" ( Citrus reticulata Blanco)的生理和形态结构变化,以及镁元素的分布特点.结果表明:１)镁缺乏使 不知火"和 椪柑"的生物量、根系活力、叶片叶绿素含量与超氧化物歧化酶(SOD)活性均显著下降,其中 不知火"的下降幅度均明显高于 椪柑" ;镁缺乏使二者叶片的过氧化物酶( POD) 活性、丙二醛(MDA)含量及叶片相对电导率显著上升,其中 不知火"的MDA 含量和叶片相对电导率显著高于 椪柑" ;在镁缺乏下 不知火"叶片的过氧化氢(CAT)活性显著升高,而?椪柑"的CAT 活性与对照差异无统计学意义;２) 不知火"和 椪柑"各部位的镁含量均随营养液中镁浓度的增加而上升,且叶片镁含量随着叶龄的增长而呈明显下降趋势,在镁缺乏下?不知火"各部位叶片镁含量均显著高于?椪柑" ;３)叶肉细胞超微结构变化显著,在镁缺乏处理下?不知火"细胞器受损程度较重,如叶绿体内基粒片层模糊、数目明显减少,淀粉粒增大,叶绿体膜与线粒体膜模糊;而?椪柑"叶绿体变形、离壁,叶绿体内基粒片层数目明显减少,淀粉粒和质体小球增多.可见,镁缺乏对 不知火"生理及形态结构的影响较?椪柑"明显.
Abstract: By using sand culture , the changes of physiological and anatomical features and the characteristics of magnesium (Mg) distribution of Citrus cultivars   shiranuhi" [ Citrus reticulata × ( C . reticulata × C .sinenesis)] and ?ponkan" ( C . reticulata) seedlings were studied in the Mg-deficient conditions . The results showed : １) The deficiency of Mg decreased significantly the plant biomass , root viability , leaf chlorophyll contents and superoxidase dismutase ( SOD) activities of both cultivars , especially for  shiranuhi" . Mg deficiency increased significantly peroxidase ( POD) activities , malondialdehyde (MDA) contents and relative conductivity of both plant leaves . Furthermore , MDA contents and relative conductivity in  shiranuhi" were significantly higher than those in   ponkan" . In addition , leaf catalase (CAT ) activity of  shiranuhi" in Mgdeficient
condition increased significantly , but it wasn't observed on   ponkan" . ２ ) Mg contents of both cultivars  leaves increased with increasing Mg concentration in solution , and they decreased obviously with the leaf aged . Furthermore ,Mg contents of   shiranuhi" were significantly higher than that of   ponkan" under Mgdeficient condition . ３) Ultrastructural observation of leaf structure and chloroplast showed that the organelle of  shiranuhi" was damaged seriously under Mg-deficient condition , which was revealed by the vague thylakoid lamella with less number ,drastic increase of starch grain volume ,and the blurred membranes of chloroplast and mitochondria .As to   ponkan" ,distortion ,plasmolysis and less grana lamella of chloroplast were observed ,but the starch grain and plastoglobuli numbers were increased . Thus , it is suggested that the influences of Mg deficiency on physiological and anatomical features were more obvious in   shiranuhi" than in  ponkan" .
出版日期: 2011-07-20
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申 燕
肖家欣
杨 慧
张绍铃

引用本文:

申 燕,肖家欣,杨 慧,张绍铃. 镁缺乏对“不知火”和“椪柑”生理指标、镁分布及叶片超微结构的影响[J]. 浙江大学学报(农业与生命科学版), 2011, 37(4): 446-452.

SHEN Yan,XIAO Jia-xin,YANG Hui,ZHANG Shao-ling. Effects of magnesium deficiency on the physiological indices, magnesium distribution and leaf ultrastructure ofCitrus cultivars  “shiranuhi” and “ponkan”. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(4): 446-452.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.04.014    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I4/446

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