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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2013, Vol. 39 Issue (5): 504-5125    DOI: 10.3785/j.issn.1008-9209.2012.12.291
农业科学     
通过条件QTL定位分析油菜不同脂肪酸组分对含油量性状的影响
王继变1,2，  陈洪成2，3， 陈玉波2,4， 张晓玉2,4， 徐海明1， 赵坚义2*
（1.浙江大学农业与生物技术学院，杭州310058；2.浙江省农业科学院作物与核技术利用研究所，杭州310021；
3.安徽农业大学生命科学学院，合肥230036；4.杭州师范大学生命与环境科学学院，杭州310036）
Molecular dissection of oil content with respect to fatty acid compositions by conditional QTL analysis in oilseed rape
WANG Jibian1,2, CHEN Hongcheng2,3, CHEN Yubo2,4, ZHANG Xiaoyu2,4, XU Haiming1, ZHAO Jianyi2*
(1. College of Agriculture &  Biotechnology, Zhejiang University, Hangzhou 310058, China; 2. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3.School of Life Sciences, Anhui Agricultural University, Hefei 230036, China; 4.College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036,China)
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摘要： 利用先前构建的Sollux/Gaoyou DH群体在4种环境下的表型结果和最新遗传图谱,通过条件和非条件QTL定位剖析油菜种子4种主要脂肪酸含量与含油量之间的遗传相关。结果显示,检测到的所有主效、上位性和环境互作QTL效应值均较小,但主效QTL都达到极显著水平（P<0.01）。分别检测到7、10、10和5个芥酸、油酸、亚油酸和亚麻酸QTL位点,其中位于A2、A7、A9和C1上的芥酸,C7和A9上的油酸和亚麻酸以及A4、A10、C6、C7和C9上的亚油酸位点为新检测出的微效QTL。条件QTL定位结果表明, OilA9、OilC2和OilC8-1受亚油酸影响较大。OilA4同时受油酸、亚麻酸和芥酸的影响, OilA1、OilA7和OilC8-2所在区间可能存在控制油酸含量的微效基因,对含油量起调控作用。另外,还检测出4个效应值更小的条件QTL新位点,进一步说明含油量性状的复杂性和难以操控性。检出的芥酸和3个18碳脂肪酸的QTL数量远多于已有在芥酸分离群体中定位到的QTL,但效应值均较小,表明在无主效芥酸等位基因分离情况下,使控制芥酸和3种18碳脂肪酸的众多微效基因得以表达。通过标志辅助聚合微效高芥酸等位基因可能对选育工业专用高芥酸油菜品种具有特殊意义,通过标志辅助选择OilA7和OilC8位点的高含油量等位基因可同步提高种子中油酸含量。
Abstract: Oil content in rapeseed is a complex quantitative trait, which is controlled by multiple genes and influenced by environments. Extensive efforts have been made in mapping of the QTL for this trait since 1995, which indicate that almost all the 19 linkage groups have found the presence of oil QTL in Brassica napus. On the other hand, a large body of QTL information for fatty acid compositions is also available because they play an important role in the accumulation of oil content. However, the genetic relationships between oil content and individual fatty acid content on QTL level still remains  poorly understood. Our main objective was to evaluate the genetic influence of four major fatty acid components on oil content in a population with no allelic segregation on two major erucic acid gene loci in oilseed rape. In present study, the previously developed doubled haploid (DH) population with Sollux / Gaoyou background (SG-population) was used for QTL identification. Both parents have high seed oil content but also high in erucic acid content. Taking the phenotypic data of oil and the four main fatty acids content from four environments, the new published SG-map was combined  to perform the QTL analysis by QTLNetwork 2.0. Results indicated that the additive effects of all mapped QTL showed small but quite significant (P<0.01). The epistatic effects and genotype by environments interaction effects were also small. By unconditional mapping analysis, 7, 10, 10 and 5 QTL were identified for erucic acid, oleic acid, linoleic acid and linolenic acid, respectively. Among them, erucic acid QTL on A2, A7, A9 and C1, and QTL for oleic acid and linolenic acid in C7 and A9 were new identified loci, while five new detected QTL for linoleic acid content were located on A4, A10, C6, C7 and C9 linkage groups. The numbers of QTL identified in present study for four fatty acid compositions are clearly more than that detected in populations with segregation for erucic acid content, indicating that the existence of allelic differentia in two major erucic acid gene loci might restrain or hide the expression of QTL with minor genetic effects.
Conditional QTL mapping indicated that OilA9, OilC2 and OilC8-1 were significantly influenced by linoleic acid, since when oil content was conditioned on linoleic acid, they showed no significant  or deeply reduced effects; OilA4 might be influenced by oleic acid, linolenic acid and erucic acid simultaneously, while oleic acid QTL or underling candidate genes may directly contribute to oil content in the loci OilA1, OilA7 and OilC8-2 because QTL for both traits were co-localized and significantly shifted the genetic effects for oil QTL after conditional mapping. In addition, four additional QTL with small effects were only identified in the conditional mapping, further demonstrating the complex genetic inheritance of oil content in B. napus.  In conclusion, by comparing unconditional and conditional QTL for oil content, the genetic relationships between oil content and fatty acid compositions on individual QTL were analyzed, which may provide valuable information for breeding varieties with high oil combining high oleic acid content. In the case of no allelic segregation on two major erucic acid gene loci, larger numbers of QTL with significant but small genetic effects were observed for all four major fatty acid compositions. Selection for high erucic acid content based on those flanking markers linked to erucic acid QTL might be special interesting for breeding high erucic acid materials for industry purpose.
出版日期: 2013-09-20
CLC:  S 565  
基金资助:

浙江省科技计划资助项目(2012C12902-1;2012C32005)；国家自然科学基金资助项目（31171180;31271608）;浙江省基金重点资助项目(Z3100592)。
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王继变1
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陈洪成2
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张晓玉2
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徐海明1
赵坚义2*

引用本文:

王继变1,2， 陈洪成2，3， 陈玉波2,4， 张晓玉2,4， 徐海明1， 赵坚义2*. 通过条件QTL定位分析油菜不同脂肪酸组分对含油量性状的影响[J]. 浙江大学学报(农业与生命科学版), 2013, 39(5): 504-5125.

WANG Jibian1,2, CHEN Hongcheng2,3, CHEN Yubo2,4, ZHANG Xiaoyu2,4, XU Haiming1, ZHAO Jianyi2*. Molecular dissection of oil content with respect to fatty acid compositions by conditional QTL analysis in oilseed rape. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(5): 504-5125.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2012.12.291    或    http://www.zjujournals.com/agr/CN/Y2013/V39/I5/504

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