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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (3): 275-279    DOI: 10.3785/j.issn.1008-9209.2011.03.006
生物科学与技术     
山西舍饲羊群羊痘病毒的分离和实验室诊断
史宗勇1,史新涛2,袁建琴1,古少鹏2,陈璋辉3,4,郑明学2
1.山西农业大学生命科学学院,山西 太谷 030801 ;2 .山西农业大学动物科技学院,山西 太谷  ;3 .浙江大学医学院公共卫生系,浙江 杭州 310058 ;4 .浙江省医学科学院,浙江 杭州 310013
Isolation and laboratory diagnosis of Capripoxvirus from goats in Shanxi Province
SHI Zhong-yong,SHI Xin-tao,YUAN Jian-qin,GU Shao-peng,CHEN Zhang-hui,ZHENG Ming-xue
1 . College of Lif e Science , Shanxi A gricultural University , Taigu , Shanxi 030801 , China ; 2 . College of Animal Science and Veterinary Medicine , Shanxi Agricultural University , Taigu , Shanxi 030801 , China ; 3 . Department of Public Health , School of Medicine , Zhej iang University , Hangzhou 310058 , China ; 4 . Zhejiang Academy of Medical Sciences , Hangzhou 310013 , China
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摘要： 为了分离舍饲羊群羊痘病毒并对其进行诊断研究,对山西舍饲羊群疑似羊痘发病羊的口唇痘状痂皮组织提取物进行细胞培养以扩增病毒,对病变细胞用苏木精-伊红染色法( hematoxylin-eosin staining , HE )观察,然后进行动物回归实验和酶联免疫吸附测定(enzyme-linked immunosorbent assay ,ELISA) .结果表明:扩增的病毒经连续培养3代后出现规律性细胞病变;用常规HE 染色可见细胞中出现由于病毒感染产生的包涵体;动物回归实验表明已分离的病毒可导致动物发生痘性病变;用ELISA检测出现显色反应,表明样品中存在与酶标抗体发生反应的病毒抗原,样品病毒浓度为6.835 IU.L-1.实验成功诊断并分离出山西舍饲羊群羊痘,为后续研究提供了理论基础.
Abstract: For clinical diagnosis , the Cap ripoxv irus from crust materials of goats with clinical sore mouth symptom from a farm in Shanxi Province was isolated .Cytopathic effect (CPE) was observed in the third passage by transfecting the virus into lamb testis ( LT ) cells . Eosinophilic intracytoplasmic inclusions were detected in the cell culture by the hematoxylin-eosin ( HE) staining . The animal showed a pox virus-infected symptom in the recurrent infection test and the result of enzyme-linked immunosorbent
assay (ELISA) showed that the concentration of the virus in the sample was 6.835 IU.L-1  , indicating the Cap ripoxv irus antigen was existed . It is indicated that the Capripoxvirus is separated and diagnosed successfully , and the results may be used for future research .
出版日期: 2011-05-20
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史宗勇,史新涛,袁建琴,古少鹏,陈璋辉,郑明学. 山西舍饲羊群羊痘病毒的分离和实验室诊断[J]. 浙江大学学报(农业与生命科学版), 2011, 37(3): 275-279.

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http://www.zjujournals.com/agr/CN/Y2011/V37/I3/275

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