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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2012, Vol. 38 Issue (2): 159-165    DOI: 10.3785/j.issn.1008-9209.2012.02.007
生物科学与技术     
鮸鱼应激蛋白STI1基因克隆及表达分析
孙悦娜， 徐田军， 石戈， 王日昕
浙江海洋学院 海洋科学学院 海洋生物资源与分子工程实验室，浙江 舟山316000
Molecular cloning and expression analysis of stress inducible  protein I gene of Miichthys miiuy.
SUN  Yue na,XU Tian jun,SHI Ge,WANG Ri xin
Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, Zhejiang 316000, China
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摘要： 根据获得的鮸鱼应激蛋白（stressinducible  protein I, STI1）基因的EST序列，克隆测序获得鮸鱼STI1基因的全长cDNA序列。鮸鱼STI1基因全长为2194 bp，包括5非翻译区31 bp，3非翻译区634 bp，开放阅读框1629 bp，编码542个氨基酸。根据氨基酸序列推测鮸鱼STI1分子中含有5个酪氨酸激酶磷酸化位点，1个多聚脯氨酸识别位点，2个核定位信号识别位点，9个TPR结构域。对物种间STI1基因比对表明，鮸鱼存在保守的半胱氨酸位点，与其他物种STI1基因的相似性在76.5%~94.3%之间。用邻接法构建的STI1基因的系统发育树表明，硬骨鱼类的STI1基因形成独立的一支，其中鮸鱼与大黄鱼的STI1基因同源性最高。荧光定量PCR结果显示，鮸鱼STI1基因的mRNA主要分布于肌肉和肾脏中。
Abstract: The full length of stressinducible  protein I (STI1) gene cDNA was cloned from Miichthys miiuy based on the obtained EST sequence using rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). The STI1 gene cDNA consisted of 2194 nucleotides including 5 untranslated regions (UTR) of 31 nucleotides, 3 UTR of 634 nucleotides and open reading frame with 1629 nucleotides encoding 542 amino acid residues. The molecular  structure of STI1 consisted of five potential casein kinase II phosphorylation sites, one polyproline stretch, two nuclear localization signals and nine tetratricopeptide repeats (TPR). The amino acid sequence of the STI1 gene shared 76.5%-94.3% identity with those of previously reported other species STI1 gene including conserved eight cysteines. Phylogenetic tree analysis based on amino acid sequences of STI1 gene generated by neighborjoining method suggested that four teleosts clustered together, and were close genetic relationship between M. miiuy and Pseudosciaena crocea. Realtime PCR showed that the STI1 gene was mainly detected in muscle and kidney.
出版日期: 2012-03-20
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孙悦娜
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引用本文:

孙悦娜， 徐田军， 石戈， 王日昕. 鮸鱼应激蛋白STI1基因克隆及表达分析[J]. 浙江大学学报(农业与生命科学版), 2012, 38(2): 159-165.

SUN Yue na,XU Tian jun,SHI Ge,WANG Ri xin. Molecular cloning and expression analysis of stress inducible  protein I gene of Miichthys miiuy.. , 2012, 38(2): 159-165.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2012.02.007    或    http://www.zjujournals.com/agr/CN/Y2012/V38/I2/159

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