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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2020, Vol. 46 Issue (6): 660-666    DOI: 10.3785/j.issn.1008-9209.2020.05.181
Reviews     
Protective effect and mechanisms of marine antifreeze protein product on sperm cryodamage in livestock
Nuo HENG1(),Yong GUO1,Yu CHEN2,Liang WANG2,Xihui SHENG1,Xiangguo WANG1,Kai XING1,Hemin NI1,Xiaolong QI1()
1.College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China
2.Beijing General Station of Animal Husbandry, Beijing 100107, China
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Abstract  

Antifreeze proteins (AFPs), a class of antifreeze substances, were initially found to bind to ice crystals in vivo, thereby contributing to inhibition of the growth of ice crystals that enabled polar fish to survive at the temperatures below freezing point. As a potential cryoprotectant, AFPs had been used for cryopreservation of germ cells, embryos and other biological samples. In this review, the application of AFPs to the cryopreservation of livestock tissues and cells in recent 10 years was summarized. The characteristics and principles of frost protection of AFPs under different biological models were discussed, and the possible influencing factors of AFPs in the cryopreservation of biological samples were analyzed. The protective effects of AFPs on sperm cryopreservation and its action mechanism were summarized, and the theoretical references were provided for the application of AFPs in cryopreservation of animal sperm. The non-toxicity and peculiar biological characteristics of AFPs in cryopreservation provided a new solution for livestock semen cryopreservation. Therefore, AFPs have a broad prospect in cryopreservation of sperm.

Key words： antifreeze protein      ice recrystallization inhibition      thermal hysteresis effect      antifreeze protectant     
Received: 18 May 2020      Published: 31 December 2020
CLC:  S 8  
Corresponding Authors: Xiaolong QI     E-mail: 1017337574@qq.com;buaqxl@126.com
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Nuo HENG
Yong GUO
Yu CHEN
Liang WANG
Xihui SHENG
Xiangguo WANG
Kai XING
Hemin NI
Xiaolong QI
Cite this article:

Nuo HENG,Yong GUO,Yu CHEN,Liang WANG,Xihui SHENG,Xiangguo WANG,Kai XING,Hemin NI,Xiaolong QI. Protective effect and mechanisms of marine antifreeze protein product on sperm cryodamage in livestock. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(6): 660-666.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2020.05.181     OR     http://www.zjujournals.com/agr/Y2020/V46/I6/660


海源性抗冻蛋白对畜禽精子冷冻损伤的保护作用及其机制

抗冻蛋白（antifreeze proteins, AFPs）是一类抗冻物质。最初发现抗冻蛋白能够与体内冰晶结合，通过抑制冰晶生长使极地鱼类能够在低于冰点的温度条件下生存。作为一种潜在的冷冻保护剂，AFPs已被用于低温保存生殖细胞和胚胎等生物样品。本文总结了近10年间AFPs在家畜组织和细胞冷冻保存技术方面的应用研究进展，探讨了AFPs在不同生物模型中抗冻保护作用的特性和原理，分析了AFPs在冷冻保存生物样本中可能产生影响的因素。同时，概述了AFPs对精子冷冻损伤的保护作用及其机制。总之，AFPs在冷冻保存中所表现出的无毒性和特有的生物学特性为家畜精液冷冻保存提供了一种新的解决方案，研究AFPs对精子的冷冻保护具有广阔的前景。


关键词： 抗冻蛋白,  抑制冰再结晶,  热滞效应,  冷冻保护剂 

抗冻蛋白

AFPs

生物样品

Biological sample

最适质量浓度

Optimal

concentration/

(μg/mL)

AFPs作用效果

Benefit of AFPs

冷冻方法

Freezing method

文献

Reference

AFPⅠ

AFPⅢ

鲤鱼精子1提高复苏后精子活力和直线速度距液氮面3 cm处蒸熏15 min[38]
AFPⅠ鲟鱼精子10提高复苏后精子活力和前向性距液氮面3 cm处蒸熏10 min[46]
DAFPs水牛精子10提高解冻后精子活力4 ℃平衡2 h后液氮蒸熏10 min[39]
AFPⅢ猪桑葚胚1提高胚胎发育质量25 ℃保存24 h[42]

AFPⅢ

FfIBP

LeIBP

鼠卵巢组织1×104降低复苏后卵巢细胞凋 亡率置于液氮中－196 ℃保存[28]
AFGP水牛精子1提高复苏后精子前向性和质膜完整性4 ℃平衡2 h后转入液氮中保存[53]
AFPⅢ牛胚胎1×104延长胚胎活力4 ℃保存10 d[54]
AFPⅢ牛卵母细胞0.5～1提高胚胎发育率置于液氮中－196 ℃保存[40]
DcAFP水牛精液10改善精子活力，提高受精率4 ℃平衡2 h后转入液氮中保存[39]
LeIBP植物细胞100提高复苏后细胞膜完整率程序化冷冻（20 ℃/min—0 ℃/min—5 ℃/min后，再0 ℃/min—40 ℃/min—1 ℃/min），液氮中保存2周[55]
AFPⅢ小鼠卵巢2×104提高复苏后卵泡完整率置于液氮中－196 ℃保存[56]
BSPs绵羊精子750提高解冻后精子运动性和顶体完整性4 ℃平衡1.5 h，再置于干冰上冷冻2 min后移入液氮中保存[57]
AFPⅢ兔精子1提高解冻后精子活力4 ℃平衡30 min后移入液氮中保存[47]
Table 1 List of AFPs cryopreserved biological samples in recent five years
Fig. 1 Schematic diagram of AFPs adsorption inhibition
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