| Animal sciences |
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| Effect of yellow capsicum extract on the lipid metabolism in mice |
| CUI Xiaoman1，YU Haining2，XU Jielan2，CHEN Zhongting3，SHEN Shengrong1* |
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1. School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058,China；
2. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China；
3. Second Affiliated Hospital, School of Medicine， Zhejiang University, Hangzhou 310009, China
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Abstract Presently, obesity is not only becoming a critical medical problem worldwide but also will cause lots of obesity-related chronic diseases as well, like hypertension, cardiovascular diseases, diabetes and so on. It becomes more and more important for current scientists to explore the effective pathway to solve the puzzle. Many researchers from different countries put much more emphasis on the anti-obesity effect of natural products. There are many studies showing that the extracts of certain parts of varied kinds of natural plants are able to inhibit the obesity through the influences on lipids metabolism, adipocytes secretion and show prevention effects on obesity through animal tests. Yellow capsicum includes functional component capsaicin which has multifunctional pharmaceutical effects. In the present study, the anti-obesity action of yellow capsicum extract (YCE) was investigated through their intervention effect on lipids metabolism in C57BL/6J mice. The total thirty-two mice were divided into four groups, including control group, obesity model group and YCE treatment groups at doses of 100 and 25 mg/kg body mass. The mice in control group were fed with normal diet and those in other groups were fed with hyperlipidic diet and YCE at different doses once a day for 40 consecutive days. All mice were weighed every five days and their body mass were recorded. In the end, the mice were killed and dissected, body fat percentage, contents of total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and leptin in serum, expression levels of TC, TG, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the liver, were all detected and analyzed. Besides, the pancrelipase (PL) level of pancreatic tissue was also detected through ELISA method, as it is an important lipase in lipids metabolism.
The results showed that, after 40 days’ treatment through hyperlipidic diet, the obesity model group was successfully built up, as they were overweighed (P<0.01) and showed a higher mass of adipose tissue (P<0.05), TC (P<0.05), TG (P<0.01) contents than the normal diet control group. Compared with obesity model group, YCE exhibited an inhibitory effect on body mass to some extent (P<0.01), fat tissues, body fat percentage (P<0.01) and Lee’s index declined (P<0.05). The contents of TC, TG and LDL of the serum in YCE treatment groups were declined (P<0.01), while there was no difference in HDL level. The liver mass (P<0.01) and its TG contents (P<0.05) in YCE treatment groups were lower than that of obesity model group. Meanwhile, TC content exhibited no significant change, on the other hand, the AST and ALT levels in the liver were normal, it showed that the YCE treatment had no side-effect on liver tissues. The PL level in YCE treatment groups was decreased (P<0.05) and it indicated that YCE might act on PL to affect lipidolysis.
From the above tests we can conclude that YCE has a significant anti-obesity effect. The changes in blood and liver indices, like TC, TG and so on, reflects its intervention on lipids metabolism. Moreover, YCE has no side-effect on liver tissues. Also YCE has a potential inhibitory effect on Pancrelipase (PL), which can be a potential target for anti-obesity therapy. Our results provide a further research evidence and basis for YCE’s deep exploration and development. Presently, obesity is not only becoming a worldwide critical medical problem but also causes lots of obesity-related chronic diseases like hypertension, cardiovascular diseases, diabetes and so on. It becomes more and more important for current scientists, exploring the effective pathway to solve the puzzle. Many researchers from different countries are putting much more emphasis on the anti-obesity effect of natural products. There are many studies showing that the extracts of certain parts of various kinds of natural plants are able to inhibit the obesity through the influences on lipid metabolism, adipocytes secretion and shows prevention effects on obesity through animal tests. Yellow capsicum has functional component capsaicin which has multifunctional pharmaceutical effects. In the present study, the anti-obesity action of yellow capsicum extract (YCE) was investigated through their intervention effect on lipids metabolism in C57BL/6J mice. Thirty-two mice were divided into four groups, including control group, obesity model group and YCE treatment groups at doses of 100 mg/kg bw and 25 mg/kg bw. The mice in control group were fed with normal diet and those in other groups were fed with
hyperlipidemic diet
and YCE at different doses once a day for 40 consecutive days. All mice were weighed every 5 days and their body mass were recorded. In the end, the mice were killed and dissected, body fat percentage, contents of total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and leptin in serum, expression levels of TC, TG, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the liver, were all detected and analyzed. Besides, the pancrelipase (PL) level of pancreatic tissue was also detected through Elisa method, as it is an important lipase in lipids metabolism. The results showed that 1) After 40 days’ treatment through hyperlipidemic diet, the obesity model group was successfully built, as they were overweighed (P<0.01) and had a higher mass of adipose tissue (P<0.05), TC (P<0.05), TG (P<0.01) contents than the normal diet control group; 2) Compared with obesity model group, YCE exhibited an inhibitory effect on body mass to some extent (P<0.01), fat tissues, body fat percentage (P<0.01) and Lee’s index declined (P<0.05); 3) The contents of TC, TG and LDL of the serum in YCE treatment groups were declined (P<0.01), while there was no difference in HDL level; 4) The liver mass (P<0.01) and its TG content (P<0.05) were lower than that of obesity model group. Meanwhile, TC content exhibited no significant changes, on the other hand, the AST and ALT levels in the liver were normal, it shows that the YCE treatment had no side-effects on liver tissues; e) The PL level in YCE treatment groups was decreased (P<0.05) and it indicated that YCE might act on PL to affect lipidolysis. From the above tests we can conclude that YCE has a significant anti-obesity effect. The changes of blood and liver indices like TC, TG and so on, reflecting its intervention on lipids metabolism. What’s more, YCE has no side-effects on liver tissues. Also YCE has a potential inhibitory effect on Pancrelipase (PL), which can be a potential target for anti-obesity therapy. Our results provide a further research evidence and basis for YCE’s deep exploration and development.
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Published: 20 May 2013
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黄樱椒提取物干预脂肪代谢的实验动物研究
研究黄樱椒提取物对营养型肥胖小鼠的脂肪代谢干预作用。将32只4周大小的C57BL/6J小鼠分为4组：空白对照组、肥胖模型组、黄樱椒提取物高剂量组及低剂量组。除空白对照组给予正常饲料以外，其余各组均喂养高脂饲料，每天灌胃给予黄樱椒提取物1次，持续灌胃40 d，每5 d称量1次体质量。实验结束，分别测定小鼠体质量、脂体比、Lee指数、血清三酰甘油（triglyceride, TG）、总胆固醇（total cholesterol, TC)、高密度脂蛋白（high-density lipoprotein, HDL）、低密度脂蛋白（low-density lipoprotein, LDL）和瘦素（leptin）含量，肝中TC、TG以及谷丙转氨酶（alanine aminotransferase, ALT）、谷草转氨酶（aspartate aminotransferase, AST）含量，以及胰脂肪酶（pancrelipase, PL）含量。与肥胖模型组比较，黄樱椒提取物能显著降低小鼠体质量（P<0.01）和脂肪总质量(P<0.01)，降低其Lee指数(P<0.05)；血清中TC含量降低(P<0.05)，TG含量降低（P<0.05），LDL含量降低（P<0.05）。不影响HDL含量，肝质量下降（P<0.01），TG含量下降(P<0.05)，对TC无影响；胰脂肪酶活性降低（P<0.05）。黄樱椒提取物对营养型肥胖小鼠具有一定的脂肪代谢干预作用，没有造成肝毒性，有进一步开发的意义。
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