Please wait a minute...
Journal of Zhejiang University (Agriculture and Life Sciences)  2013, Vol. 39 Issue (3): 343-350    DOI: 10.3785/j.issn.1008-9209.2012.09.301
Animal sciences     
Effect of soy isoflavones on intraepithelial lymphocytes, goblet cells and long leptin receptor in intestinal of rats
TANG Xiuying1,  CHEN Zhengli1,2,3*, LUO Qihui2,3, ZHANG Xiaolong1
(1. Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya′an, Sichuan 625014, China
; 2. Experimental Animal Engineering Center / Provenance Source Depot of Macaque for National Experiment,  Sichuan Agricultural University, Ya′an,Sichuan 625014, China; 3. Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China)
Download: HTML (   PDF(4311KB)
Export: BibTeX | EndNote (RIS)      

Abstract  Soy isoflavones (SIF) are  a research focus due to their potential health effects. Some studies have shown that it could modulate immune function and prevention of chronic diseases, but until now it remains controversial. Thus, the aim of the present study is  to investigate the effect of high-fat diet induced obesity on male immune parameters. Numerous studies have reported that immunomodulatory activity of intestinal mucosal barrier was related to intraepithelial lymphocytes (IEL) and goblet cells (GC). Leptin is secreted almost exclusively by adipocytes. Previous researches demonstrated that plasma leptin levels in ovariectomized rats was lower than that in estradiol-treated animals. Moreover, there was a tissue-specific regulation of the leptin receptor (OB-Rb), with less sensitivity to leptin in adipose tissue when the time of treatment or the dose of  estradiol administered increased. The metabolites of soy isoflavones showed  structural and functional similarities to estradiol. Available data indicated that soy isoflavones inhibited the leptin secretion, and thus influenced  the expression of OB-Rb. The study was divided into two steps. In the first experiment, male Sprague-Dawley rats were fed with a high sugar and fat diet (HSF) or basal  diet for eight weeks. Food intake and animal mass were recorded weekly. In the second experiment, 40 selected obese rats were randomly divided into four groups with 10 rats in each group and were fed with soy isoflavones (group Ⅰ 0 mg/kg, group Ⅱ 50 mg/kg,  group Ⅲ 150 mg/kg and group IV 450 mg/kg) and 10 control rats (group V) were fed with basal  diet for four weeks. Soy isoflavones was gavaged with 0.5% sodium carboxymethyl cellulose solutions respectively.
At the end of the second experiment, rats were slightly anesthetized with sodium pentobarbital ip (40 mg/kg), and  intestinal was  taken and fixed in 10% neutral buffered formalin, processed and trimmed, embedded in paraffin. IEL and GC in the villus epithelium were evaluated in these rats, as well as the OB-Rb expression by immunohistochemistry staining. In the first experiment, the body mass of the HSF-fed animals were significantly increased after  eight weeks  and the obesity module was succeeded. In the second experiment, the results showed that the structure of mucous epithelium, intraepithelial lymphocyte and the goblet cells were normal in the intestinal. The intraepithelial lymphocytes of rats were mainly small lymphocytes and located around the epithelial basement membrane. Goblet cells distributed in the intestinal mucous epithelial lamina, and OB-Rb positive cells distributed in the intestinal mucous epithelial lamina and lamina propria, respectively. Compared with 0 mg/kg-SIF group, there was a tendency that IEL in the villus epithelium moved inward intestinal entoderm in SIF-treated rats. Quantitative analysis of IEL and GC in the villus epithelium showed the number of GC in duodenum, jejunum, ileum, colon had a increasing tendency from the forepart to the back of intestinal.The number of IEL and GC in the epithelium in the 450 mg/kg-SIF group were increased significantly (P<0.05), and there was significant difference in numbers of GC between the control and other groups. The changes of OB-Rb were relatively irregular in each part of the intestinal. The OB-Rb expression also increased significantly (P<0.05) in intestinal mucous membrane in the 450 mg/kg-SIF group. IEL and GC may play a very important role in the local mucosa immunity. The low number of IEL and GC showed that obesity may impair the  immune function of rats. The results showed that SIF can affect mucosal immunity by increasing the number of IEL and GC. The OB-Rb expression of SIF-treated rats was significantly increased compared with untreated ones. We deduced that SIF contains a number of biologically active components with potential immunomodulatory activity, and SIF could modulate intestinal mucosal immunity of rats.
 


Published: 20 May 2013
CLC:  S 852.31  
Cite this article:

TANG Xiuying1, CHEN Zhengli1,2,3*, LUO Qihui2,3, ZHANG Xiaolong1. Effect of soy isoflavones on intraepithelial lymphocytes, goblet cells and long leptin receptor in intestinal of rats. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(3): 343-350.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2012.09.301     OR     http://www.zjujournals.com/agr/Y2013/V39/I3/343


大豆异黄酮对大鼠肠道上皮内淋巴细胞、杯状细胞及瘦素长型受体的影响

大豆异黄酮具有广泛的生理作用,其对免疫系统的影响近年来成为一个焦点。为了研究大豆异黄酮对肠黏膜屏障结构的重要组成部分(上皮内淋巴细胞、杯状细胞)及免疫调节因子瘦素受体(长型)的影响,本实验采用高脂饲料喂饲大鼠,建立肥胖模型;然后将筛选出的肥胖大鼠分别灌胃剂量为
对照(I:0 mg/kg)、低(Ⅱ:50 mg/kg)、中(Ⅲ:150 mg/kg)、高剂量(Ⅳ:450 mg/kg)的大豆异黄酮,并设置基础对照组(Ⅴ:溶媒为0.5%羧甲基纤维素钠),
连续4周用
HE、PAS染色观察小肠上皮内淋巴细胞、杯状细胞数量和分布变化;用免疫组织化学SABC法测定瘦素受体(长型)水平。结果表明:大鼠上皮内淋巴细胞以小型淋巴细胞为主,主要分布于上皮的基底膜附近;大鼠杯状细胞分布在肠黏膜上皮层;瘦素受体(长型)阳性细胞分布于黏膜层。应用大豆异黄酮高剂量组较对照组大鼠的肠黏膜上皮内淋巴细胞数量和杯状细胞的数量明显增加,且有向肠内层移动趋势,同时肠黏膜瘦素受体(长型)水平升高,差异有统计学意义(P<0.05)。表明大豆异黄酮在一定程度上可促进大鼠小肠黏膜屏障结构趋于完整并提高其免疫功能。
No related articles found!