Comparison and analysis of antimicrobial resistance of <i>Escherichia coli </i>and <i>Enterococcus</i> isolated from animals in Jinhua City and Taizhou City of Zhejiang Province

doi:10.3785/j.issn.1008-9209.2020.11.121

Journal of Zhejiang University (Agriculture and Life Sciences)

2021, Vol. 47

Issue (5): 647-659 DOI: 10.3785/j.issn.1008-9209.2020.11.121

Animal sciences & veterinary medicine

Comparison and analysis of antimicrobial resistance of Escherichia coli and Enterococcus isolated from animals in Jinhua City and Taizhou City of Zhejiang Province

Biao TANG^1,²(

),Yun HAO^1,²,Jiahui LIN³,Jingge WANG^2,⁴,Xiaofeng JI^1,²,Mingrong QIAN^1,²,Hua YANG^1,²(

)

^1.State Key Laboratory of Quality Safety and Risk Factor Prevention and Control of Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
^2.Institute of Agro-products Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
^3.College of Life Science and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
^4.College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China

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Abstract

To investigate the antimicrobial resistance of bacteria from animals in Jinhua City and Taizhou City of Zhejiang Province in May of 2020, a total of 284 anal swab samples were randomly collected from seven livestock and poultry farms in the two cities, respectively. Escherichia coli and Enterococcus were isolated by selective culture media and were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The minimal inhibitory concentration (MIC) was determined by broth microdilution method. The results showed that the antimicrobial resistance rate of E. coli to tetracycline was the highest (87.5%), followed by sulfamethoxazole (81.2%), while the antimicrobial resistance rate to colistin was the lowest (0%) in Jinhua City. In addition, the antimicrobial resistance rate of E. coli to sulfamethoxazole was the highest (97.1%), and the resistance rates to meropenem and colistin were both 0% in Taizhou City. The antimicrobial resistance rate of Enterococcus to sulfamethoxazole was 100% in both cities, followed by tamoxifen (98.6%). No amoxicillin/clavulanate and vancomycin resistant Enterococcus was found in the two cities. In terms of the overall antimicrobial resistance rate, MIC distribution and antimicrobial resistance pattern, the level of antimicrobial resistance in Taizhou was higher than that in Jinhua. In conclusion, through monitoring and comparing the level of antimicrobial resistance in livestock and poultry breeding in two cities, it is found that the levels of antimicrobial resistance of E. coli and Enterococcus from animals are high and different. Therefore, continuous surveillance of antimicrobial resistance dynamics is essential through veterinary antibiotics reduction initiatives by agricultural authorities.

Key words： livestock and poultry Escherichia coli Enterococcus antimicrobial resistance Jinhua City Taizhou City

Received: 12 November 2020 Published: 27 October 2021

CLC:

S 852.6

Corresponding Authors: Hua YANG E-mail: tb_411@163.com;yanghua@zaas.ac.cn

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	Biao TANG
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	Jiahui LIN
	Jingge WANG
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	Mingrong QIAN
	Hua YANG

Cite this article:

Biao TANG,Yun HAO,Jiahui LIN,Jingge WANG,Xiaofeng JI,Mingrong QIAN,Hua YANG. Comparison and analysis of antimicrobial resistance of Escherichia coli and Enterococcus isolated from animals in Jinhua City and Taizhou City of Zhejiang Province. Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(5): 647-659.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2020.11.121 OR http://www.zjujournals.com/agr/Y2021/V47/I5/647

浙江省2市动物源大肠埃希菌及肠球菌耐药性比较和分析

为了解2020年浙江省金华市和台州市动物源细菌耐药情况，于2020年5月分别从这2市7个畜禽养殖场随机采集畜禽肛拭子样品284份，使用选择性培养基分离大肠埃希菌和肠球菌，通过基质辅助激光解吸电离飞行时间质谱（matrix-assisted laser desorption ionization time-of-flight mass spectrometry, MALDI-TOF-MS）对其进行鉴定，并使用微量肉汤稀释法检测这2种细菌的最小抑菌浓度（minimal inhibitory concentration, MIC）。结果显示：金华市大肠埃希菌对四环素的耐药率最高（87.5%），其次是对磺胺异噁唑（81.2%），对黏菌素的耐药率最低（0%）；台州市大肠埃希菌对磺胺异噁唑的耐药率最高（97.1%），对美罗培南和黏菌素的耐药率均为0%。金华和台州2市肠球菌对磺胺异噁唑耐药率均为100%，其次是对泰妙菌素，均为98.6%，2市未检测到对奥格门汀和万古霉素耐药的肠球菌。此外，从整体耐药率、MIC分布和耐药谱型上看，台州市的细菌耐药性水平比金华市的高。总之，通过对2个地级市的畜禽养殖中细菌耐药性水平进行监测和对比发现，2市的动物源大肠埃希菌和肠球菌耐药水平较高且存在差异。因此，通过农业主管部门的兽药减量化行动，持续监测耐药性动态变化十分必要。

关键词： 畜禽, 大肠埃希菌, 肠球菌, 耐药性, 金华市, 台州市

Fig. 1 Identification spectra of quality control strains by MALDI-TOF-MS

Fig. 2 Comparison of antimicrobial resistance rates of E. coli isolated from Jinhua City and Taizhou CityAMP: Ampicillin; A/C: Amoxicillin/clavulanate; GEM: Gentamicin; SPT: Spectinomycin; TET: Tetracycline; FFC: Florfenicol; SF: Sulfisoxazole; SXT: Trimethoprim/sulfamethoxazole; CEF: Ceftiofur; CAZ: Ceftazidime; ENR: Enrofloxacin; OFL: Ofloxacin; MEM: Meropenem; CL: Colistin.

Fig. 3 MIC distributions of 16 antibiotics to E. coli isolated from Jinhua City and Taizhou CityAMP: Ampicillin; A/C: Amoxicillin/clavulanate; GEM: Gentamicin; SPT: Spectinomycin; TET: Tetracycline; FFC: Florfenicol; SF: Sulfisoxazole; SXT: Trimethoprim/sulfamethoxazole; CEF: Ceftiofur; CAZ: Ceftazidime; ENR: Enrofloxacin; OFL: Ofloxacin; MEM: Meropenem; APR: Apramycin; CL: Colistin; MEQ: Mequindox.

Fig. 4 Comparison of antimicrobial resistance rates of Enterococcus isolated from Jinhua City and Taizhou CityPEN:Ampicillin; A/C: Amoxicillin/clavulanate; ERY: Erythromycin; ENR: Enrofloxacin; SF: Sulfisoxazole; VAN: Vancomycin; DOX: Doxycycline; FFC: Florfenicol; TIA: Tiamulin; TIL: Tilmicosin; LZD: Linezolid.

Fig. 5 MIC distributions of 27 antibioticsfor Enterococcus isolated from Jinhua City and Taizhou CityPEN: Penicillin; A/C: Amoxicillin/clavulanate; ERY: Erythromycin; CLI: Clindamycin; ENR: Enrofloxacin; OFL: Ofloxacin; CEF: Ceftiofur; CFX: Cefoxitin; SF: Sulfisoxazole; OXA: Oxacillin; VAN: Vancomycin; SXT: Trimethoprim/sulfamethoxazole; DOX: Doxycycline; FFC: Florfenicol; TIA: Tiamulin; TIL: Tilmicosin; GEM: Gentamicin; LZD: Linezolid; BAC: Bacitracin; TET: Tetracycline; KIT: Kitasamycin; FLA: Flavomycin; EDC: Enramycin; QCT: Quinocetone; NOS: Nosiheptide; AVI: Avilamycin; VGM: Virginiamycin.

Table 2 Antimicrobial resistance patterns of E. coli isolated from Taizhou City

Table 3 Antimicrobial resistance patterns of Enterococcus isolated from Jinhua City

Table 4 Antimicrobial resistance patterns of Enterococcus isolated from Taizhou City


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