We analyzed the yield, quality and disease resistance traits of fresh maize cultivars from regional testing in Zhejiang Province from 2010 to 2016. The results showed that the yield, quality and disease resistance traits of the 165 fresh maize cultivars were significantly different between cultivars and years. In the yield trait, the variation coefficients of sweet corn varied from 3.7% (growth period) to 17.8% (ear height), whereas the variation coefficients of waxy corn varied from 2.2% (growth period) to 11.5% (1 000-grain mass). In the quality trait, the variation coefficients of sweet corn varied from 2.6% (total score) to 6.4% (flavor), whereas the variation coefficients of waxy corn were from 1.9% (smell) to 8.4% (flavor). All the examined maize cultivars exhibited low resistance to corn borer, and no high resistance cultivars were found. Moreover, their resistance to southern leaf blight was low, but the percentage of cultivars with the southern leaf blight resistance had a tendency of increase. By contrast, the total level of resistance to northern leaf blight was generally good. No cultivars with high resistance to sheath blight were found, but the resistance to sheath blight was improved in recent years. The total level of resistance to stem rot was good, and furthermore the percentage of stem rot resistant cultivars was increased continually. These results suggest that the resistance level to main diseases and insect pests of fresh maize cultivars in Zhejiang Province is relatively low, but has a growing trend; the comprehensive resistance level is also constantly improved. Most of the cultivars possess a high-yield trait (≈1×104 kg/hm²), medium quality (total score of 84-86) and medium resistance level. Combined the analysis of regional test data with the actual production of Zhejiang Province, some specific objectives of fresh maize breeding are put forward in this study.